Evaluating the effect of selected medicinal plant extracts and their synergistic effect with metronidazole against entamoeba histolytica
Other Title(s)
تقييم أثر بعض النباتات الطبية و مدى تأزرها مع المترونايدازول على مسبب الزحار الاميبى (انتاميبا هستوليتكا)
Dissertant
Thesis advisor
Comitee Members
al-Sharif, Fadl Akram
al-Rayyan, Abd al-Nasir Ismail
University
Islamic University
Faculty
Faculty of Science
Department
Department of Biological Science
University Country
Palestine (Gaza Strip)
Degree
Master
Degree Date
2014
English Abstract
Introduction: Intestinal infection with Entamoeba histolytica is an important cause of diarrhoea world-wide especially where sanitation conditions are poor.
Medicinal plants have played a significant role in various traditional systems of medications including intestinal infections caused by E.
histolytica.
Objectives: To investigate the antiamoebic activity of pomegranate, castor and thyme aqueous and alcoholic extracts, in addition to thyme oil and pomergnate juice against E.
histolytica and to determine the synergistic effect of these plant extracts with the antibiotic metronidazole.
Methods: Three medicinal plants pomegranate (Punica granatum), castor (Ricinus communis L) and thyme (Thymus vulgaris L) were used in this study.
Some of these plants were bought from fruit's market in Gaza and some were collected from some regions in Gaza; each plant was dried then extracted according to standard extraction method using Soxhlet apparatus.
E.
histolytica was cultured in a modified diphasic liver infusion agar medium in vitro conditions to study the effect of these plant extracts on E.
histolytica and their synergestic effect with the antibiotic metronidazole.
The type of E.
histolytica was confirmed using PCR.
Results: Successful cultures of E.
histolytica were obtained.
All the plant materials used in this study possessed antiamoebic activity in vitro with different levels except the aqueous extract of R.
communis leaves which also was the only one didn‟t show synergistic effect with metronidazole at tested concentrations.
Alcoholic extract of P.
granatum pulp had the strongest antiamoebic activity with MIC of < 5 mg/ml while the aqueous extract of T.
vulgaris leaves had the weakest antiamoebic activity with a MIC of < 20 mg/ml
Main Subjects
Topics
No. of Pages
104
Table of Contents
Table of contents.
Abstract.
Abstract in Arabic.
Chapter One : Introduction.
Chapter Two : Literature review.
Chapter Three : Materials and methods.
Chapter Four : Results.
Chapter Five : Discussion.
References.
American Psychological Association (APA)
Dardunah, Zuhayr Wadi Yusuf. (2014). Evaluating the effect of selected medicinal plant extracts and their synergistic effect with metronidazole against entamoeba histolytica. (Master's theses Theses and Dissertations Master). Islamic University, Palestine (Gaza Strip)
https://search.emarefa.net/detail/BIM-533885
Modern Language Association (MLA)
Dardunah, Zuhayr Wadi Yusuf. Evaluating the effect of selected medicinal plant extracts and their synergistic effect with metronidazole against entamoeba histolytica. (Master's theses Theses and Dissertations Master). Islamic University. (2014).
https://search.emarefa.net/detail/BIM-533885
American Medical Association (AMA)
Dardunah, Zuhayr Wadi Yusuf. (2014). Evaluating the effect of selected medicinal plant extracts and their synergistic effect with metronidazole against entamoeba histolytica. (Master's theses Theses and Dissertations Master). Islamic University, Palestine (Gaza Strip)
https://search.emarefa.net/detail/BIM-533885
Language
English
Data Type
Arab Theses
Record ID
BIM-533885