Characterization of Phenoloxidase Enzyme from Giant Clam (Tridcana maxima)‎ in Gulf of Aqaba in Red Sea

العناوين الأخرى

دراسة إنزيم الفينول اوكسيديز في البطلينوس العملاق في خليج العقبة في البحر الأحمر

مقدم أطروحة جامعية

Abu Shattal, Saqr Sulayman

مشرف أطروحة جامعية

Aladaileh, Salim
Atrooz, Umar M.

أعضاء اللجنة

al-Trawnih, Khalid
Quran, Salih
Rawashidah, Ibrahim

الجامعة

جامعة مؤتة

الكلية

كلية العلوم

القسم الأكاديمي

قسم الأحياء

دولة الجامعة

الأردن

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2014

الملخص الإنجليزي

Phenoloxidase (PO) activity was studied in Giant Clam (Tridcana maxima).

The PO activity was measured by monitoring the absorbance at 490 nm using a microplate reader.

As in other molluscs, PO was found to exist as a proenzyme (proPO) in hemocytes.

Inhibition studies (EDTA, CPIC, PMSF and HBHZ) confirmed the proPO activating system of Giant Clam is a proteinase cascade in which Ca2+ dependent serine proteinases proteolytically convert proPO into active PO.

Activated PO was found to be a tyrosinase-like enzyme that is responsible for both monophenolase and diphenolase activity.

The bifunctional PO had the same affinity for the monophenol, hydroquinine monomethyl ether (4HA) (Km = 11.03 mM) and the diphenol, L-DOPA (Km = 11.91mM).

Maximum enzyme activity was evident at 45 °C, pH 8 and 45ppt for L-DOPA and maximum enzyme activity was evident at 30 °C, pH 9 and (30 to 40) ppt for 4HA. The results of this study were shown that phenoloxidase activity in the Giant Clam (Tridcana maxima) does provide a reliable indicator of stress.

التخصصات الرئيسية

الأحياء
علم الحيوان

الموضوعات

عدد الصفحات

42

قائمة المحتويات

Table of contents.

Abstract.

Abstract in Arabic.

Chapter One : Theoretical background.

Chapter Two : Literature review.

Chapter Three : Materials and methods.

Chapter Four : Results, discussion and recommendation.

References.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Abu Shattal, Saqr Sulayman. (2014). Characterization of Phenoloxidase Enzyme from Giant Clam (Tridcana maxima) in Gulf of Aqaba in Red Sea. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-556652

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Abu Shattal, Saqr Sulayman. Characterization of Phenoloxidase Enzyme from Giant Clam (Tridcana maxima) in Gulf of Aqaba in Red Sea. (Master's theses Theses and Dissertations Master). Mutah University. (2014).
https://search.emarefa.net/detail/BIM-556652

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Abu Shattal, Saqr Sulayman. (2014). Characterization of Phenoloxidase Enzyme from Giant Clam (Tridcana maxima) in Gulf of Aqaba in Red Sea. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-556652

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-556652