Characterization of Phenoloxidase Enzyme from Giant Clam (Tridcana maxima) in Gulf of Aqaba in Red Sea
Other Title(s)
دراسة إنزيم الفينول اوكسيديز في البطلينوس العملاق في خليج العقبة في البحر الأحمر
Dissertant
Thesis advisor
Aladaileh, Salim
Atrooz, Umar M.
Comitee Members
al-Trawnih, Khalid
Quran, Salih
Rawashidah, Ibrahim
University
Mutah University
Faculty
Faculty of Science
Department
Department of Biological Sciences
University Country
Jordan
Degree
Master
Degree Date
2014
English Abstract
Phenoloxidase (PO) activity was studied in Giant Clam (Tridcana maxima).
The PO activity was measured by monitoring the absorbance at 490 nm using a microplate reader.
As in other molluscs, PO was found to exist as a proenzyme (proPO) in hemocytes.
Inhibition studies (EDTA, CPIC, PMSF and HBHZ) confirmed the proPO activating system of Giant Clam is a proteinase cascade in which Ca2+ dependent serine proteinases proteolytically convert proPO into active PO.
Activated PO was found to be a tyrosinase-like enzyme that is responsible for both monophenolase and diphenolase activity.
The bifunctional PO had the same affinity for the monophenol, hydroquinine monomethyl ether (4HA) (Km = 11.03 mM) and the diphenol, L-DOPA (Km = 11.91mM).
Maximum enzyme activity was evident at 45 °C, pH 8 and 45ppt for L-DOPA and maximum enzyme activity was evident at 30 °C, pH 9 and (30 to 40) ppt for 4HA. The results of this study were shown that phenoloxidase activity in the Giant Clam (Tridcana maxima) does provide a reliable indicator of stress.
Main Subjects
Topics
No. of Pages
42
Table of Contents
Table of contents.
Abstract.
Abstract in Arabic.
Chapter One : Theoretical background.
Chapter Two : Literature review.
Chapter Three : Materials and methods.
Chapter Four : Results, discussion and recommendation.
References.
American Psychological Association (APA)
Abu Shattal, Saqr Sulayman. (2014). Characterization of Phenoloxidase Enzyme from Giant Clam (Tridcana maxima) in Gulf of Aqaba in Red Sea. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-556652
Modern Language Association (MLA)
Abu Shattal, Saqr Sulayman. Characterization of Phenoloxidase Enzyme from Giant Clam (Tridcana maxima) in Gulf of Aqaba in Red Sea. (Master's theses Theses and Dissertations Master). Mutah University. (2014).
https://search.emarefa.net/detail/BIM-556652
American Medical Association (AMA)
Abu Shattal, Saqr Sulayman. (2014). Characterization of Phenoloxidase Enzyme from Giant Clam (Tridcana maxima) in Gulf of Aqaba in Red Sea. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-556652
Language
English
Data Type
Arab Theses
Record ID
BIM-556652
