Antimicrobial activity of some native plants of south Jordan

العناوين الأخرى

الأنشطة المضادة للبكتريا من بعض النباتات في جنوب الأردن

مقدم أطروحة جامعية

Abu Darwish, Muayyad Mahdi

مشرف أطروحة جامعية

al-Tarawinah, Khalid Ahmad

الجامعة

جامعة مؤتة

الكلية

كلية العلوم

القسم الأكاديمي

قسم الأحياء

دولة الجامعة

الأردن

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2014

الملخص الإنجليزي

In the current research, MeOH extracts of Artemisia monosperma, Crataegus azarolus and Cleome droserifolia were studied to explore antioxidant activity and antimicrobial activity against five bacterial strains (Bacillus subtilis , Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus and Micrococcus luteus ).

The highest extract yield obtained from A.

monosperma , the extract weight of A.

monosperma reach about 5.7 g which act 23% of the used dry weight .

The plants extract of A.

monosperma , C.

azarolus and C.

droserifolia showed a noticed inhibition activity against B.

subtilis, K.

pneumonia, S.

aureus and M.

luteus while no inhibition zones indicated on E.

coli.

According to minimal inhibitory concentration (MIC) test ; the lowest MIC values belong to A.

monosperma extract against most used bacterial strains and the highest MIC values reported to Crataegus azarolus against most tested bacterial strains .

Antioxidant activity was measured using two protocols (DPPH and FRAP).

In DPPH assay which used to detect radical scavenging activity of plant extract, the smallest IC50 values that exhibit highest scavenging activity recorded for A.

monosperma and C.

droserifolia extracts (IC50 = 0.05 and 0.12 mg/mL, respectively).

In FRAP assay which indicate ferric reducing antioxidant power A.

monosperma has the highest activity (5.93 mg Trolox/g of plant extract) followed by C.

droserifolia and C.

azarolus ( 4.49 and 1.82 mg Trolox/g of plant extract, respectively).

Total phenol content (TPC) of plant extracts showed that C.

azarolus has lowest amount of total phenolics (133 mg GAE/g) while C.

droserifolia extract contain 480 mg GAE/g and 1200 mg GAE/g in A.

monosperma extract.

A strong correlation expressed between TPC and antioxidant activity of plant extract , since the largest TPC value was found in A.

monosperma extract which also show the highest antioxidant activity while the smallest TPC value refer to C.

azarolus extract which exhibit lowest antioxidant activity

التخصصات الرئيسية

الأحياء

الموضوعات

• النباتات الطبية
• الأدوية
• الأردن

عدد الصفحات

40

قائمة المحتويات

• APA
• MLA
• AMA

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-561633