Antimicrobial activity of some native plants of south Jordan
Other Title(s)
الأنشطة المضادة للبكتريا من بعض النباتات في جنوب الأردن
Dissertant
Thesis advisor
University
Mutah University
Faculty
Faculty of Science
Department
Department of Biological Sciences
University Country
Jordan
Degree
Master
Degree Date
2014
English Abstract
In the current research, MeOH extracts of Artemisia monosperma, Crataegus azarolus and Cleome droserifolia were studied to explore antioxidant activity and antimicrobial activity against five bacterial strains (Bacillus subtilis , Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus and Micrococcus luteus ).
The highest extract yield obtained from A.
monosperma , the extract weight of A.
monosperma reach about 5.7 g which act 23% of the used dry weight .
The plants extract of A.
monosperma , C.
azarolus and C.
droserifolia showed a noticed inhibition activity against B.
subtilis, K.
pneumonia, S.
aureus and M.
luteus while no inhibition zones indicated on E.
coli.
According to minimal inhibitory concentration (MIC) test ; the lowest MIC values belong to A.
monosperma extract against most used bacterial strains and the highest MIC values reported to Crataegus azarolus against most tested bacterial strains .
Antioxidant activity was measured using two protocols (DPPH and FRAP).
In DPPH assay which used to detect radical scavenging activity of plant extract, the smallest IC50 values that exhibit highest scavenging activity recorded for A.
monosperma and C.
droserifolia extracts (IC50 = 0.05 and 0.12 mg/mL, respectively).
In FRAP assay which indicate ferric reducing antioxidant power A.
monosperma has the highest activity (5.93 mg Trolox/g of plant extract) followed by C.
droserifolia and C.
azarolus ( 4.49 and 1.82 mg Trolox/g of plant extract, respectively).
Total phenol content (TPC) of plant extracts showed that C.
azarolus has lowest amount of total phenolics (133 mg GAE/g) while C.
droserifolia extract contain 480 mg GAE/g and 1200 mg GAE/g in A.
monosperma extract.
A strong correlation expressed between TPC and antioxidant activity of plant extract , since the largest TPC value was found in A.
monosperma extract which also show the highest antioxidant activity while the smallest TPC value refer to C.
azarolus extract which exhibit lowest antioxidant activity
Main Subjects
Topics
No. of Pages
40
Table of Contents
Table of contents.
Abstract.
Abstract in Arabic.
Chapter One : Theoretical background.
Chapter Two : Literature review.
Chapter Three : Materials and methods.
Chapter Four : Results, discussion and recommendation.
References.
American Psychological Association (APA)
Abu Darwish, Muayyad Mahdi. (2014). Antimicrobial activity of some native plants of south Jordan. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-561633
Modern Language Association (MLA)
Abu Darwish, Muayyad Mahdi. Antimicrobial activity of some native plants of south Jordan. (Master's theses Theses and Dissertations Master). Mutah University. (2014).
https://search.emarefa.net/detail/BIM-561633
American Medical Association (AMA)
Abu Darwish, Muayyad Mahdi. (2014). Antimicrobial activity of some native plants of south Jordan. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-561633
Language
English
Data Type
Arab Theses
Record ID
BIM-561633
