Genetic diversity of some tomato lycopersicon esculentum mill varieties in Iraq using simple sequence repeat (SSR) markers
العناوين الأخرى
التنوع الوراثي لبعض أصناف الطماطة (Lycopersicon esculentum Mill.) بإستخدام واسمات التتابع المتكرر البسيط (SSRs)
المؤلفون المشاركون
al-Tamimi, Attyaf Jamil Thamir
al-Sadi, Ali Hmood
Abbas, Muhsin Jallab
المصدر
al-Kufa University Journal for Biology
العدد
المجلد 7، العدد 1 (30 يونيو/حزيران 2015)16ص.
الناشر
جامعة الكوفة كلية العلوم قسم علوم الحياة
تاريخ النشر
2015-06-30
دولة النشر
العراق
عدد الصفحات
16
التخصصات الرئيسية
الموضوعات
الملخص AR
This study was conducted to evaluate the genetic diversity among 19 tomato varieties (determinate and indeterminate) cultivated in Iraq using polymerase chain reaction based DNA marker (PCR based DNA markers Simple Sequence Repeats (SSRs).
To achieve PCR reactions ,total genomic DNA was isolated from fresh leaves (2 weeks old).
The average yields of DNA were in the range of 100-295 ng/μl with a purity ranging between 1.8-1.9.
A total of 21 alleles were detected among the tested varieties using 5 SSRs loci distributed on 4 chromosomes of tomato.
The molecular size of bands obtained from amplification of SSR products ranged from 121 to 247 bp.
Alleles ranged from one in (Tom 8-9, Tom 41-42 and Tom 67-68 loci) to twelve in Tom 49-50 locus.
The values of heterozygosity for each locus ranged between 0.63 for Tom 31-32 and 0.89 for Tom 49-50 with a mean value of 0.30.
The polymorphic information content (PIC) values for the SSR loci ranged from 0.45 in Tom 31-32 to 0.58 in Tom 49-50 loci with an average of 0.21.
Each one of (Tom 8-9, Tom 41-42 and Tom 67-68 loci) produce 0.0 value for both heterozygosity and PIC.
The study revealed that, The lowest genetic distance was (0.3244) between varieties Kenanh (lane5) and Warda (lane10), while,the highest genetic distance was (0.9177) between varieties Helam (32 to 91%) among the tested varieties.
The analysis of the obtained results from genetic distances and Neighbor-joining dendrogram (unrooted tree) revealed that, the 19 tested tomato varieties can be grouped into two major groups: first cluster include 9 varieties distributed in two subgroups.The second major cluster include 10 varieties which in turn divided into two subgroups.
الملخص EN
This study was conducted to evaluate the genetic diversity among 19 tomato varieties (determinate and indeterminate) cultivated in Iraq using polymerase chain reaction based DNA marker (PCR based DNA markers Simple Sequence Repeats (SSRs).
To achieve PCR reactions ,total genomic DNA was isolated from fresh leaves (2 weeks old).
The average yields of DNA were in the range of 100-295 ng/μl with a purity ranging between 1.8-1.9.
A total of 21 alleles were detected among the tested varieties using 5 SSRs loci distributed on 4 chromosomes of tomato.
The molecular size of bands obtained from amplification of SSR products ranged from 121 to 247 bp.
Alleles ranged from one in (Tom 8-9, Tom 41-42 and Tom 67-68 loci) to twelve in Tom 49-50 locus.
The values of heterozygosity for each locus ranged between 0.63 for Tom 31-32 and 0.89 for Tom 49-50 with a mean value of 0.30.
The polymorphic information content (PIC) values for the SSR loci ranged from 0.45 in Tom 31-32 to 0.58 in Tom 49-50 loci with an average of 0.21.
Each one of (Tom 8-9, Tom 41-42 and Tom 67-68 loci) produce 0.0 value for both heterozygosity and PIC.
The study revealed that, The lowest genetic distance was (0.3244) between varieties Kenanh (lane5) and Warda (lane10), while,the highest genetic distance was (0.9177) between varieties Helam (32 to 91%) among the tested varieties.
The analysis of the obtained results from genetic distances and Neighbor-joining dendrogram (unrooted tree) revealed that, the 19 tested tomato varieties can be grouped into two major groups: first cluster include 9 varieties distributed in two subgroups.The second major cluster include 10 varieties which in turn divided into two subgroups.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Tamimi, Attyaf Jamil Thamir& al-Sadi, Ali Hmood& Abbas, Muhsin Jallab. 2015. Genetic diversity of some tomato lycopersicon esculentum mill varieties in Iraq using simple sequence repeat (SSR) markers. al-Kufa University Journal for Biology،Vol. 7, no. 1.
https://search.emarefa.net/detail/BIM-594215
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Tamimi, Attyaf Jamil Thamir…[et al.]. Genetic diversity of some tomato lycopersicon esculentum mill varieties in Iraq using simple sequence repeat (SSR) markers. al-Kufa University Journal for Biology Vol. 7, no. 1 (2015).
https://search.emarefa.net/detail/BIM-594215
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Tamimi, Attyaf Jamil Thamir& al-Sadi, Ali Hmood& Abbas, Muhsin Jallab. Genetic diversity of some tomato lycopersicon esculentum mill varieties in Iraq using simple sequence repeat (SSR) markers. al-Kufa University Journal for Biology. 2015. Vol. 7, no. 1.
https://search.emarefa.net/detail/BIM-594215
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-594215
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر