![](/images/graphics-bg.png)
Genetic diversity of some tomato lycopersicon esculentum mill varieties in Iraq using simple sequence repeat (SSR) markers
Other Title(s)
التنوع الوراثي لبعض أصناف الطماطة (Lycopersicon esculentum Mill.) بإستخدام واسمات التتابع المتكرر البسيط (SSRs)
Joint Authors
al-Tamimi, Attyaf Jamil Thamir
al-Sadi, Ali Hmood
Abbas, Muhsin Jallab
Source
al-Kufa University Journal for Biology
Issue
Vol. 7, Issue 1 (30 Jun. 2015)16 p.
Publisher
University of Kufa Faculty of Science Department of live Sciences
Publication Date
2015-06-30
Country of Publication
Iraq
No. of Pages
16
Main Subjects
Topics
Abstract AR
This study was conducted to evaluate the genetic diversity among 19 tomato varieties (determinate and indeterminate) cultivated in Iraq using polymerase chain reaction based DNA marker (PCR based DNA markers Simple Sequence Repeats (SSRs).
To achieve PCR reactions ,total genomic DNA was isolated from fresh leaves (2 weeks old).
The average yields of DNA were in the range of 100-295 ng/μl with a purity ranging between 1.8-1.9.
A total of 21 alleles were detected among the tested varieties using 5 SSRs loci distributed on 4 chromosomes of tomato.
The molecular size of bands obtained from amplification of SSR products ranged from 121 to 247 bp.
Alleles ranged from one in (Tom 8-9, Tom 41-42 and Tom 67-68 loci) to twelve in Tom 49-50 locus.
The values of heterozygosity for each locus ranged between 0.63 for Tom 31-32 and 0.89 for Tom 49-50 with a mean value of 0.30.
The polymorphic information content (PIC) values for the SSR loci ranged from 0.45 in Tom 31-32 to 0.58 in Tom 49-50 loci with an average of 0.21.
Each one of (Tom 8-9, Tom 41-42 and Tom 67-68 loci) produce 0.0 value for both heterozygosity and PIC.
The study revealed that, The lowest genetic distance was (0.3244) between varieties Kenanh (lane5) and Warda (lane10), while,the highest genetic distance was (0.9177) between varieties Helam (32 to 91%) among the tested varieties.
The analysis of the obtained results from genetic distances and Neighbor-joining dendrogram (unrooted tree) revealed that, the 19 tested tomato varieties can be grouped into two major groups: first cluster include 9 varieties distributed in two subgroups.The second major cluster include 10 varieties which in turn divided into two subgroups.
Abstract EN
This study was conducted to evaluate the genetic diversity among 19 tomato varieties (determinate and indeterminate) cultivated in Iraq using polymerase chain reaction based DNA marker (PCR based DNA markers Simple Sequence Repeats (SSRs).
To achieve PCR reactions ,total genomic DNA was isolated from fresh leaves (2 weeks old).
The average yields of DNA were in the range of 100-295 ng/μl with a purity ranging between 1.8-1.9.
A total of 21 alleles were detected among the tested varieties using 5 SSRs loci distributed on 4 chromosomes of tomato.
The molecular size of bands obtained from amplification of SSR products ranged from 121 to 247 bp.
Alleles ranged from one in (Tom 8-9, Tom 41-42 and Tom 67-68 loci) to twelve in Tom 49-50 locus.
The values of heterozygosity for each locus ranged between 0.63 for Tom 31-32 and 0.89 for Tom 49-50 with a mean value of 0.30.
The polymorphic information content (PIC) values for the SSR loci ranged from 0.45 in Tom 31-32 to 0.58 in Tom 49-50 loci with an average of 0.21.
Each one of (Tom 8-9, Tom 41-42 and Tom 67-68 loci) produce 0.0 value for both heterozygosity and PIC.
The study revealed that, The lowest genetic distance was (0.3244) between varieties Kenanh (lane5) and Warda (lane10), while,the highest genetic distance was (0.9177) between varieties Helam (32 to 91%) among the tested varieties.
The analysis of the obtained results from genetic distances and Neighbor-joining dendrogram (unrooted tree) revealed that, the 19 tested tomato varieties can be grouped into two major groups: first cluster include 9 varieties distributed in two subgroups.The second major cluster include 10 varieties which in turn divided into two subgroups.
American Psychological Association (APA)
al-Tamimi, Attyaf Jamil Thamir& al-Sadi, Ali Hmood& Abbas, Muhsin Jallab. 2015. Genetic diversity of some tomato lycopersicon esculentum mill varieties in Iraq using simple sequence repeat (SSR) markers. al-Kufa University Journal for Biology،Vol. 7, no. 1.
https://search.emarefa.net/detail/BIM-594215
Modern Language Association (MLA)
al-Tamimi, Attyaf Jamil Thamir…[et al.]. Genetic diversity of some tomato lycopersicon esculentum mill varieties in Iraq using simple sequence repeat (SSR) markers. al-Kufa University Journal for Biology Vol. 7, no. 1 (2015).
https://search.emarefa.net/detail/BIM-594215
American Medical Association (AMA)
al-Tamimi, Attyaf Jamil Thamir& al-Sadi, Ali Hmood& Abbas, Muhsin Jallab. Genetic diversity of some tomato lycopersicon esculentum mill varieties in Iraq using simple sequence repeat (SSR) markers. al-Kufa University Journal for Biology. 2015. Vol. 7, no. 1.
https://search.emarefa.net/detail/BIM-594215
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-594215