![](/images/graphics-bg.png)
Detection of 16s ribosomal RNA methylation in extended-spectrum β-lactamase-producing Klebsiella pneumoniae clinical isolates from Baghdad Hospitals
المؤلفون المشاركون
al-Kadmi, Isra M. S.
Musafir, Hadil K.
Ali, Marwah Hasan Abd
Sajid, Sawsan
المصدر
Iraqi Journal of Biotechnology
العدد
المجلد 14، العدد 1 (30 يونيو/حزيران 2015)، ص ص. 53-62، 10ص.
الناشر
جامعة بغداد معهد الهندسة الوراثية و التقنيات الإحيائية للدراسات العليا
تاريخ النشر
2015-06-30
دولة النشر
العراق
عدد الصفحات
10
التخصصات الرئيسية
الموضوعات
الملخص AR
Fifty-eight Clinical isolates of Klebsiella pneumoniae were collected from patients at Al-Kindey teaching hospital, Al-Kadhymia teaching hospital, Ibn-Albalady hospital and Al-Imam-Ali hospital in Baghdad from Urinary tract infection (UTI) patient.
These isolates were diagnosed using different morphological and biochemical test followed by the complementary API 20E, isolates all screened for antibiotic resistance by rapid detection of β-lactamases production method and their minimum inhibitory concentrations(MICs) were conducted by polymerase chain reaction assay.
Detection of blaCTX –M gene was performed and highly resistant isolates (n=17) were selected to determine aminoglycoside resistance mediated by methylation 16S rRNA that combined with the detection of blaCTX –M gene responsible for Extended Spectrum β-lactamase (ESβLs) production.
Seven 16S rRNA methylase genes were amplified, the ArmA, RmtA, RmtB, RmtC, RmtD, RmtF and npmA beside amplifying blaCTX –M gene.
This study explained the physically link between blaCTX-M gene (ESβLs) production among Klebsiella pneumoniae in correlation with 16S rRNA methylatio
الملخص EN
Fifty-eight Clinical isolates of Klebsiella pneumoniae were collected from patients at Al-Kindey teaching hospital, Al-Kadhymia teaching hospital, Ibn-Albalady hospital and Al-Imam-Ali hospital in Baghdad from Urinary tract infection (UTI) patient.
These isolates were diagnosed using different morphological and biochemical test followed by the complementary API 20E, isolates all screened for antibiotic resistance by rapid detection of β-lactamases production method and their minimum inhibitory concentrations(MICs) were conducted by polymerase chain reaction assay.
Detection of blaCTX–M gene was performed and highly resistant isolates (n = 17) were selected to determine aminoglycoside resistance mediated by methylation 16S rRNA that combined with the detection of blaCTX–M gene responsible for Extended Spectrum β-lactamase (ESβLs) production.
Seven 16S rRNA methylase genes were amplified, the ArmA, RmtA, RmtB, RmtC, RmtD, RmtF and npmA beside amplifying blaCTX–M gene.
This study explained the physically link between blaCTX-M gene (ESβLs) production among Klebsiella pneumoniae in correlation with 16S rRNA methylation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Kadmi, Isra M. S.& Musafir, Hadil K.& Ali, Marwah Hasan Abd& Sajid, Sawsan. 2015. Detection of 16s ribosomal RNA methylation in extended-spectrum β-lactamase-producing Klebsiella pneumoniae clinical isolates from Baghdad Hospitals. Iraqi Journal of Biotechnology،Vol. 14, no. 1, pp.53-62.
https://search.emarefa.net/detail/BIM-596132
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Kadmi, Isra M. S.…[et al.]. Detection of 16s ribosomal RNA methylation in extended-spectrum β-lactamase-producing Klebsiella pneumoniae clinical isolates from Baghdad Hospitals. Iraqi Journal of Biotechnology Vol. 14, no. 1 (2015), pp.53-62.
https://search.emarefa.net/detail/BIM-596132
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Kadmi, Isra M. S.& Musafir, Hadil K.& Ali, Marwah Hasan Abd& Sajid, Sawsan. Detection of 16s ribosomal RNA methylation in extended-spectrum β-lactamase-producing Klebsiella pneumoniae clinical isolates from Baghdad Hospitals. Iraqi Journal of Biotechnology. 2015. Vol. 14, no. 1, pp.53-62.
https://search.emarefa.net/detail/BIM-596132
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 60-62
رقم السجل
BIM-596132
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)