Detection of 16s ribosomal RNA methylation in extended-spectrum β-lactamase-producing Klebsiella pneumoniae clinical isolates from Baghdad Hospitals
Joint Authors
al-Kadmi, Isra M. S.
Musafir, Hadil K.
Ali, Marwah Hasan Abd
Sajid, Sawsan
Source
Iraqi Journal of Biotechnology
Issue
Vol. 14, Issue 1 (30 Jun. 2015), pp.53-62, 10 p.
Publisher
University of Baghdad Genetic Engineering and Biotechnology for Postgraduate Studies
Publication Date
2015-06-30
Country of Publication
Iraq
No. of Pages
10
Main Subjects
Topics
Abstract AR
Fifty-eight Clinical isolates of Klebsiella pneumoniae were collected from patients at Al-Kindey teaching hospital, Al-Kadhymia teaching hospital, Ibn-Albalady hospital and Al-Imam-Ali hospital in Baghdad from Urinary tract infection (UTI) patient.
These isolates were diagnosed using different morphological and biochemical test followed by the complementary API 20E, isolates all screened for antibiotic resistance by rapid detection of β-lactamases production method and their minimum inhibitory concentrations(MICs) were conducted by polymerase chain reaction assay.
Detection of blaCTX –M gene was performed and highly resistant isolates (n=17) were selected to determine aminoglycoside resistance mediated by methylation 16S rRNA that combined with the detection of blaCTX –M gene responsible for Extended Spectrum β-lactamase (ESβLs) production.
Seven 16S rRNA methylase genes were amplified, the ArmA, RmtA, RmtB, RmtC, RmtD, RmtF and npmA beside amplifying blaCTX –M gene.
This study explained the physically link between blaCTX-M gene (ESβLs) production among Klebsiella pneumoniae in correlation with 16S rRNA methylatio
Abstract EN
Fifty-eight Clinical isolates of Klebsiella pneumoniae were collected from patients at Al-Kindey teaching hospital, Al-Kadhymia teaching hospital, Ibn-Albalady hospital and Al-Imam-Ali hospital in Baghdad from Urinary tract infection (UTI) patient.
These isolates were diagnosed using different morphological and biochemical test followed by the complementary API 20E, isolates all screened for antibiotic resistance by rapid detection of β-lactamases production method and their minimum inhibitory concentrations(MICs) were conducted by polymerase chain reaction assay.
Detection of blaCTX–M gene was performed and highly resistant isolates (n = 17) were selected to determine aminoglycoside resistance mediated by methylation 16S rRNA that combined with the detection of blaCTX–M gene responsible for Extended Spectrum β-lactamase (ESβLs) production.
Seven 16S rRNA methylase genes were amplified, the ArmA, RmtA, RmtB, RmtC, RmtD, RmtF and npmA beside amplifying blaCTX–M gene.
This study explained the physically link between blaCTX-M gene (ESβLs) production among Klebsiella pneumoniae in correlation with 16S rRNA methylation.
American Psychological Association (APA)
al-Kadmi, Isra M. S.& Musafir, Hadil K.& Ali, Marwah Hasan Abd& Sajid, Sawsan. 2015. Detection of 16s ribosomal RNA methylation in extended-spectrum β-lactamase-producing Klebsiella pneumoniae clinical isolates from Baghdad Hospitals. Iraqi Journal of Biotechnology،Vol. 14, no. 1, pp.53-62.
https://search.emarefa.net/detail/BIM-596132
Modern Language Association (MLA)
al-Kadmi, Isra M. S.…[et al.]. Detection of 16s ribosomal RNA methylation in extended-spectrum β-lactamase-producing Klebsiella pneumoniae clinical isolates from Baghdad Hospitals. Iraqi Journal of Biotechnology Vol. 14, no. 1 (2015), pp.53-62.
https://search.emarefa.net/detail/BIM-596132
American Medical Association (AMA)
al-Kadmi, Isra M. S.& Musafir, Hadil K.& Ali, Marwah Hasan Abd& Sajid, Sawsan. Detection of 16s ribosomal RNA methylation in extended-spectrum β-lactamase-producing Klebsiella pneumoniae clinical isolates from Baghdad Hospitals. Iraqi Journal of Biotechnology. 2015. Vol. 14, no. 1, pp.53-62.
https://search.emarefa.net/detail/BIM-596132
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 60-62
Record ID
BIM-596132
