Isolation of uropathogens from pediatric associated UTI, with special focus on the detection of Proteus vulgaris

الملخص EN

The study was conducted in AL-Kadhumia hospital for children, AL-Kadhumia city / Baghdad, through period April-July 2014.

Mid–stream urine of 75 patients were cultured.

Out of these 67 (89.33 %) patients` urine were positive for bacteriological culture.

The distribution of infection was 50.7 % infants aged 2-9 months and 46.3 % children aged 1-4 years.

Total of six bacterial species isolated were E.coli 35 (52.2 %), Enterobacter spp.

16 (23.9 %), Proteus spp.11 (14.9 %), Klebsialle spp.

3(4.7 %), Pseudomonas spp.

1 (1.5 %) and Staphylococcus spp.

1(1.5 %).

The isolated organisms were identified based on conventional bacteriological and biochemical analyses and were characterized.

PCR technique was used to detect Proteus vulgaris by using species–specific primers Urease C, the results of primer urease C observe 3 isolate were positive.

Rapid diagnosis of the pathogen in a clinical sample is always very important.