Isolation of uropathogens from pediatric associated UTI, with special focus on the detection of Proteus vulgaris
Joint Authors
al-Sadi, Basimah Q. Hasan
Muhaysin, Siham Hamil
Kazim, Sana Jasim
Source
Iraqi Journal of Biotechnology
Issue
Vol. 14, Issue 1 (30 Jun. 2015), pp.77-84, 8 p.
Publisher
University of Baghdad Genetic Engineering and Biotechnology for Postgraduate Studies
Publication Date
2015-06-30
Country of Publication
Iraq
No. of Pages
8
Main Subjects
Topics
Abstract AR
The study was conducted in AL-Kadhumia hospital for children, AL-Kadhumia city / Baghdad, through period April-July 2014.
Mid –stream urine of 75 patients were cultured.
Out of these 67(89.33%) patients` urine were positive for bacteriological culture.
The distribution of infection was 50.7% infants aged 2-9 months and 46.3% children aged 1-4 years.
Total of six bacterial species isolated were E.coli 35(52.2%), Enterobacter spp.
16(23.9%), Proteus spp.11 (14.9%), Klebsialle spp.
3(4.7%), Pseudomonas spp.
1 (1.5 %) and Staphylococcus spp.
1(1.5 %).
The isolated organisms were identified based on conventional bacteriological and biochemical analyses and were characterized.
PCR technique was used to detect Proteus vulgaris by using species – specific primers Urease C, the results of primer urease C observe 3 isolate were positive.
Rapid diagnosis of the pathogen in a clinical sample is always very important
Abstract EN
The study was conducted in AL-Kadhumia hospital for children, AL-Kadhumia city / Baghdad, through period April-July 2014.
Mid–stream urine of 75 patients were cultured.
Out of these 67 (89.33 %) patients` urine were positive for bacteriological culture.
The distribution of infection was 50.7 % infants aged 2-9 months and 46.3 % children aged 1-4 years.
Total of six bacterial species isolated were E.coli 35 (52.2 %), Enterobacter spp.
16 (23.9 %), Proteus spp.11 (14.9 %), Klebsialle spp.
3(4.7 %), Pseudomonas spp.
1 (1.5 %) and Staphylococcus spp.
1(1.5 %).
The isolated organisms were identified based on conventional bacteriological and biochemical analyses and were characterized.
PCR technique was used to detect Proteus vulgaris by using species–specific primers Urease C, the results of primer urease C observe 3 isolate were positive.
Rapid diagnosis of the pathogen in a clinical sample is always very important.
American Psychological Association (APA)
al-Sadi, Basimah Q. Hasan& Kazim, Sana Jasim& Muhaysin, Siham Hamil. 2015. Isolation of uropathogens from pediatric associated UTI, with special focus on the detection of Proteus vulgaris. Iraqi Journal of Biotechnology،Vol. 14, no. 1, pp.77-84.
https://search.emarefa.net/detail/BIM-596152
Modern Language Association (MLA)
al-Sadi, Basimah Q. Hasan…[et al.]. Isolation of uropathogens from pediatric associated UTI, with special focus on the detection of Proteus vulgaris. Iraqi Journal of Biotechnology Vol. 14, no. 1 (2015), pp.77-84.
https://search.emarefa.net/detail/BIM-596152
American Medical Association (AMA)
al-Sadi, Basimah Q. Hasan& Kazim, Sana Jasim& Muhaysin, Siham Hamil. Isolation of uropathogens from pediatric associated UTI, with special focus on the detection of Proteus vulgaris. Iraqi Journal of Biotechnology. 2015. Vol. 14, no. 1, pp.77-84.
https://search.emarefa.net/detail/BIM-596152
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 83-84
Record ID
BIM-596152
