Reactive oxygen species induced by enterobacteriaceae caused DNA fragmentation in human uroepithelial cells
مقدم أطروحة جامعية
al-Saffar, Usamah Bassim Abd al-Khaliq
مشرف أطروحة جامعية
أعضاء اللجنة
الجامعة
جامعة بغداد
الكلية
كلية العلوم
القسم الأكاديمي
قسم علوم الحياة
دولة الجامعة
العراق
الدرجة العلمية
ماجستير
تاريخ الدرجة العلمية
2010
الملخص الإنجليزي
Three hundred mid-stream urine specimens were collected from 300 Urinary Tract Infections (UTI) patients who admitted to Al-Yarmok hospital in Baghdad city at the period 1st February to 1st April 2009.
One hundred and thirty isolates were obtained from mid-stream urines specimens included: E.
coli (63.84%), Klebsiella spp.(23.07%), (K.
oxytoca 16.15%, K.
planticola 6.92% ).
Enterobacter aerogenes (6.19%) and Proteus spp.
(6.92%) (P.
vulgaris 4.61%, P.
mirabilis 2.31%).
They were identified according to the cultural and biochemical properties.
Patients were divided into five groups ( A, B, C, D and E) according to the pus cells level in their urine specimens.
Moreover, the type and prevalence of bacterial infection in pus cells in each studied group were detected.
E.
coli performed the highest percentage (61.36%) in all studied groups, particularly group A.
Also the study included the assessment of uropathogens to induce ROS which measured by using malonadialdehyde (MDA) method.
The results showed that the level of ROS was significantly (P<0.05) increased according to the level of pus cells.
Therefore, group E showed high level of ROS (9.08 nmol/l) in comparison with other groups in this study.
On the other hand, the ability of uropathogens to induce ROS was determined.
E.
coli isolates particularly E.
coli19 showed a putative efficiency for induction ROS (11.62 nmol/l).
In contrast, K.planticola Kp4 exhibited the lowest level of ROS (3.14 nmol/l).
The correlation between ROS level and fragmentation DNA in nuclei (n) and mitochondria (mt) was estimated.
In general, increasing level of ROS induced either nDNA or mtDNA fragmentation, nDNA fragmentation in group E was achieved high percentage (65.14%) and associated with a high level of ROS (9.08 nmol/l).
Similar results were obtained from mtDNA fragmentation.
Nevertheless, ROS significantly (P<0.05) affected mtDNA more than nDNA.
التخصصات الرئيسية
عدد الصفحات
97
قائمة المحتويات
Table of contents.
Abstract.
Abstract in Arabic.
Chapter One : Introduction and review of literature.
Chapter Two : Materials and methods.
Chapter Three : Result and discussion.
Conclusions and recommendations.
References.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Saffar, Usamah Bassim Abd al-Khaliq. (2010). Reactive oxygen species induced by enterobacteriaceae caused DNA fragmentation in human uroepithelial cells. (Master's theses Theses and Dissertations Master). University of Baghdad, Iraq
https://search.emarefa.net/detail/BIM-597372
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Saffar, Usamah Bassim Abd al-Khaliq. Reactive oxygen species induced by enterobacteriaceae caused DNA fragmentation in human uroepithelial cells. (Master's theses Theses and Dissertations Master). University of Baghdad. (2010).
https://search.emarefa.net/detail/BIM-597372
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Saffar, Usamah Bassim Abd al-Khaliq. (2010). Reactive oxygen species induced by enterobacteriaceae caused DNA fragmentation in human uroepithelial cells. (Master's theses Theses and Dissertations Master). University of Baghdad, Iraq
https://search.emarefa.net/detail/BIM-597372
لغة النص
الإنجليزية
نوع البيانات
رسائل جامعية
رقم السجل
BIM-597372
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر