Reactive oxygen species induced by enterobacteriaceae caused DNA fragmentation in human uroepithelial cells

مقدم أطروحة جامعية

al-Saffar, Usamah Bassim Abd al-Khaliq

مشرف أطروحة جامعية

al-Hassani, Hayfa Hadi

أعضاء اللجنة

al-Musawi, Zayn S.

الجامعة

جامعة بغداد

الكلية

كلية العلوم

القسم الأكاديمي

قسم علوم الحياة

دولة الجامعة

العراق

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2010

الملخص الإنجليزي

Three hundred mid-stream urine specimens were collected from 300 Urinary Tract Infections (UTI) patients who admitted to Al-Yarmok hospital in Baghdad city at the period 1st February to 1st April 2009.

One hundred and thirty isolates were obtained from mid-stream urines specimens included: E.

coli (63.84%), Klebsiella spp.(23.07%), (K.

oxytoca 16.15%, K.

planticola 6.92% ).

Enterobacter aerogenes (6.19%) and Proteus spp.

(6.92%) (P.

vulgaris 4.61%, P.

mirabilis 2.31%).

They were identified according to the cultural and biochemical properties.

Patients were divided into five groups ( A, B, C, D and E) according to the pus cells level in their urine specimens.

Moreover, the type and prevalence of bacterial infection in pus cells in each studied group were detected.

E.

coli performed the highest percentage (61.36%) in all studied groups, particularly group A.

Also the study included the assessment of uropathogens to induce ROS which measured by using malonadialdehyde (MDA) method.

The results showed that the level of ROS was significantly (P<0.05) increased according to the level of pus cells.

Therefore, group E showed high level of ROS (9.08 nmol/l) in comparison with other groups in this study.

On the other hand, the ability of uropathogens to induce ROS was determined.

E.

coli isolates particularly E.

coli19 showed a putative efficiency for induction ROS (11.62 nmol/l).

In contrast, K.planticola Kp4 exhibited the lowest level of ROS (3.14 nmol/l).

The correlation between ROS level and fragmentation DNA in nuclei (n) and mitochondria (mt) was estimated.

In general, increasing level of ROS induced either nDNA or mtDNA fragmentation, nDNA fragmentation in group E was achieved high percentage (65.14%) and associated with a high level of ROS (9.08 nmol/l).

Similar results were obtained from mtDNA fragmentation.

Nevertheless, ROS significantly (P<0.05) affected mtDNA more than nDNA.

التخصصات الرئيسية

الأحياء

عدد الصفحات

97

قائمة المحتويات

• APA
• MLA
• AMA

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-597372