Reactive oxygen species induced by enterobacteriaceae caused DNA fragmentation in human uroepithelial cells
Dissertant
al-Saffar, Usamah Bassim Abd al-Khaliq
Thesis advisor
Comitee Members
University
University of Baghdad
Faculty
College of Science
Department
Department of Biology
University Country
Iraq
Degree
Master
Degree Date
2010
English Abstract
Three hundred mid-stream urine specimens were collected from 300 Urinary Tract Infections (UTI) patients who admitted to Al-Yarmok hospital in Baghdad city at the period 1st February to 1st April 2009.
One hundred and thirty isolates were obtained from mid-stream urines specimens included: E.
coli (63.84%), Klebsiella spp.(23.07%), (K.
oxytoca 16.15%, K.
planticola 6.92% ).
Enterobacter aerogenes (6.19%) and Proteus spp.
(6.92%) (P.
vulgaris 4.61%, P.
mirabilis 2.31%).
They were identified according to the cultural and biochemical properties.
Patients were divided into five groups ( A, B, C, D and E) according to the pus cells level in their urine specimens.
Moreover, the type and prevalence of bacterial infection in pus cells in each studied group were detected.
E.
coli performed the highest percentage (61.36%) in all studied groups, particularly group A.
Also the study included the assessment of uropathogens to induce ROS which measured by using malonadialdehyde (MDA) method.
The results showed that the level of ROS was significantly (P<0.05) increased according to the level of pus cells.
Therefore, group E showed high level of ROS (9.08 nmol/l) in comparison with other groups in this study.
On the other hand, the ability of uropathogens to induce ROS was determined.
E.
coli isolates particularly E.
coli19 showed a putative efficiency for induction ROS (11.62 nmol/l).
In contrast, K.planticola Kp4 exhibited the lowest level of ROS (3.14 nmol/l).
The correlation between ROS level and fragmentation DNA in nuclei (n) and mitochondria (mt) was estimated.
In general, increasing level of ROS induced either nDNA or mtDNA fragmentation, nDNA fragmentation in group E was achieved high percentage (65.14%) and associated with a high level of ROS (9.08 nmol/l).
Similar results were obtained from mtDNA fragmentation.
Nevertheless, ROS significantly (P<0.05) affected mtDNA more than nDNA.
Main Subjects
No. of Pages
97
Table of Contents
Table of contents.
Abstract.
Abstract in Arabic.
Chapter One : Introduction and review of literature.
Chapter Two : Materials and methods.
Chapter Three : Result and discussion.
Conclusions and recommendations.
References.
American Psychological Association (APA)
al-Saffar, Usamah Bassim Abd al-Khaliq. (2010). Reactive oxygen species induced by enterobacteriaceae caused DNA fragmentation in human uroepithelial cells. (Master's theses Theses and Dissertations Master). University of Baghdad, Iraq
https://search.emarefa.net/detail/BIM-597372
Modern Language Association (MLA)
al-Saffar, Usamah Bassim Abd al-Khaliq. Reactive oxygen species induced by enterobacteriaceae caused DNA fragmentation in human uroepithelial cells. (Master's theses Theses and Dissertations Master). University of Baghdad. (2010).
https://search.emarefa.net/detail/BIM-597372
American Medical Association (AMA)
al-Saffar, Usamah Bassim Abd al-Khaliq. (2010). Reactive oxygen species induced by enterobacteriaceae caused DNA fragmentation in human uroepithelial cells. (Master's theses Theses and Dissertations Master). University of Baghdad, Iraq
https://search.emarefa.net/detail/BIM-597372
Language
English
Data Type
Arab Theses
Record ID
BIM-597372
