The effect of ethanolic extract of Curcuma longa rhizomes on two types of leukemic cell lines and karyotyping of human lymphocytes treated by nickel
العناوين الأخرى
تأثير المستخلص الكحولي لرايزومات نبات الكركم Curcuma longa على خطين خلويين من مرض ابيضاض الدم و طبعة النواة للخلايا المعاملة بالنيكل
مقدم أطروحة جامعية
مشرف أطروحة جامعية
Shawkat, Muayyad Sabri
al-Rikabi, Abd al-Amir Nasir Ghalub
الجامعة
جامعة بغداد
الكلية
كلية العلوم
القسم الأكاديمي
قسم التقانة الإحيائية
دولة الجامعة
العراق
الدرجة العلمية
ماجستير
تاريخ الدرجة العلمية
2011
الملخص الإنجليزي
In order to investigate the active compounds of Curcuma longa rhizomes, crude extract was prepared by using ethanol.
Chemical detection of crude extract was performed and showed positive result for alkaloids, tannins, glycosides, phenols, Terpens, flavonoids, cumarines and resins while showed negative result for Saponins and Steroids.
Reversed high performance liquid chromatography carried out for the detection and estimation of curcumin in the crude extract.
Different concentrations of C.
longa ethanolic crud extract and chemotherapeutic drug (Melphalan) (500, 250, 100, 50, 25, and 10 μg /ml), were used to detect the cytotxicity to two leukemic cell lines which were acute myeloid leukemia (U937) and acute lymphoblastic leukemia (Molt4), The cytotoxic inhibition effects of the Curcuma longa crude extract was highly significant ( P< 0.01) at concentrations 25, 50, 250 and 500 μg /ml while no-significant differences recorded at concentrations 10 and 100 μg /ml if compared with cytotoxic effect of melphalan against U937 cell line., on the other hand, the cytotoxic effects of crude extract on the cell line Molt4 showed significant differences (P< 0.01) at concentrations 25, 50, 100, 250 and 500 μg /ml and non-significant differences at if 10 μg /ml if compared with the cytotoxic effect of melphalan.
The results showed that the IC50 of crude extract was 19.34μg/ml and the IC50 of melphalan was 20.75μg/ml for the (U937) cell line while the IC50 of crude extract was 46.58μg/ml and the IC50 of melphalan was 54.48μg/ml for (Molt4) cell line.
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Summary The results of the cytogenetic study of the effect of C.
longa crude extract and standard curcumin on the normal human lymphocyte blood cells after exposure to nickel chloride (NiCl2) revealed that both of them reduced the genotoxic effect of the (NiCl2), furthermore, karyotyping was performed by using Metasystem Ikaros which showed that genotoxic effect of nickel chloride appeared as aberration in chromosome number 18.
While lymphocytes treated with C.
longa crude extract or standard curcumin showed no chromosomal aberration, at the same time no chromosomal aberration were observed after treatment with combination of NiCl2 and crude extract or combination of NiCl2 and standard curcumin which indicated the ability of standard curcumin and C.longa crude extract to prevent the effect of nickel chloride.
التخصصات الرئيسية
عدد الصفحات
99
قائمة المحتويات
Table of contents.
Abstract.
Introduction.
Chapter One : Literature review.
Chapter Two : Materials and methods.
Chapter Three : Results and discussion.
Chapter Four : Conclusions and recommendations.
References.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hashim, Farah Jabbar. (2011). The effect of ethanolic extract of Curcuma longa rhizomes on two types of leukemic cell lines and karyotyping of human lymphocytes treated by nickel. (Master's theses Theses and Dissertations Master). University of Baghdad, Iraq
https://search.emarefa.net/detail/BIM-600737
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hashim, Farah Jabbar. The effect of ethanolic extract of Curcuma longa rhizomes on two types of leukemic cell lines and karyotyping of human lymphocytes treated by nickel. (Master's theses Theses and Dissertations Master). University of Baghdad. (2011).
https://search.emarefa.net/detail/BIM-600737
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hashim, Farah Jabbar. (2011). The effect of ethanolic extract of Curcuma longa rhizomes on two types of leukemic cell lines and karyotyping of human lymphocytes treated by nickel. (Master's theses Theses and Dissertations Master). University of Baghdad, Iraq
https://search.emarefa.net/detail/BIM-600737
لغة النص
الإنجليزية
نوع البيانات
رسائل جامعية
رقم السجل
BIM-600737
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر