The effect of ethanolic extract of Curcuma longa rhizomes on two types of leukemic cell lines and karyotyping of human lymphocytes treated by nickel

Other Title(s)

تأثير المستخلص الكحولي لرايزومات نبات الكركم Curcuma longa على خطين خلويين من مرض ابيضاض الدم و طبعة النواة للخلايا المعاملة بالنيكل

Dissertant

Hashim, Farah Jabbar

Thesis advisor

Shawkat, Muayyad Sabri
al-Rikabi, Abd al-Amir Nasir Ghalub

University

University of Baghdad

Faculty

College of Science

Department

Biotechnology Department

University Country

Iraq

Degree

Master

Degree Date

2011

English Abstract

In order to investigate the active compounds of Curcuma longa rhizomes, crude extract was prepared by using ethanol.

Chemical detection of crude extract was performed and showed positive result for alkaloids, tannins, glycosides, phenols, Terpens, flavonoids, cumarines and resins while showed negative result for Saponins and Steroids.

Reversed high performance liquid chromatography carried out for the detection and estimation of curcumin in the crude extract.

Different concentrations of C.

longa ethanolic crud extract and chemotherapeutic drug (Melphalan) (500, 250, 100, 50, 25, and 10 μg /ml), were used to detect the cytotxicity to two leukemic cell lines which were acute myeloid leukemia (U937) and acute lymphoblastic leukemia (Molt4), The cytotoxic inhibition effects of the Curcuma longa crude extract was highly significant ( P< 0.01) at concentrations 25, 50, 250 and 500 μg /ml while no-significant differences recorded at concentrations 10 and 100 μg /ml if compared with cytotoxic effect of melphalan against U937 cell line., on the other hand, the cytotoxic effects of crude extract on the cell line Molt4 showed significant differences (P< 0.01) at concentrations 25, 50, 100, 250 and 500 μg /ml and non-significant differences at if 10 μg /ml if compared with the cytotoxic effect of melphalan.

The results showed that the IC50 of crude extract was 19.34μg/ml and the IC50 of melphalan was 20.75μg/ml for the (U937) cell line while the IC50 of crude extract was 46.58μg/ml and the IC50 of melphalan was 54.48μg/ml for (Molt4) cell line.

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Summary The results of the cytogenetic study of the effect of C.

longa crude extract and standard curcumin on the normal human lymphocyte blood cells after exposure to nickel chloride (NiCl2) revealed that both of them reduced the genotoxic effect of the (NiCl2), furthermore, karyotyping was performed by using Metasystem Ikaros which showed that genotoxic effect of nickel chloride appeared as aberration in chromosome number 18.

While lymphocytes treated with C.

longa crude extract or standard curcumin showed no chromosomal aberration, at the same time no chromosomal aberration were observed after treatment with combination of NiCl2 and crude extract or combination of NiCl2 and standard curcumin which indicated the ability of standard curcumin and C.longa crude extract to prevent the effect of nickel chloride.

Main Subjects

Biology

No. of Pages

99

Table of Contents

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Language

English

Data Type

Arab Theses

Record ID

BIM-600737