Isolation and identification of clinical Pseudomonas aeruginosa producing exotoxin a and studying its toxic effect in mice

العناوين الأخرى

عزل و تشخيص بكتريا Pseudomonas aeruginosa المرضية المنتجة للسم الخارجي A و دراسة تأثيره السمي في الفئران

مقدم أطروحة جامعية

Nafi, Sarah Qusayy

مشرف أطروحة جامعية

al-Tai, Maha Fakhri
Hamzah, Subhi Jawad

الجامعة

جامعة بغداد

الكلية

كلية العلوم

القسم الأكاديمي

قسم التقانة الإحيائية

دولة الجامعة

العراق

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2012

الملخص الإنجليزي

Forty-five pathogenic bacterial isolates were identified as P.

aeruginosa which isolated from seventy-five patients suffering from various infections, twenty-five isolates were found producing exotoxin A as detected by specific ELISA kit.

Accordingly, three isolates (PA10, PA11 and PA15) were selected according to their productivity and site of infections as detected by ELISA kit.

Exotoxins A were partially purified using 90% saturation of ammonium sulphate then DEAE-cellulose column.

Isolate PA15 was found able to produce 32.08ng/ml of active exotoxin A (ETA) with a protein of about 0.063 mg/ml.

Molecular weight of the toxin was determined to be 65,000 Dalton.

Subsequently, three dose of ETA (125, 250 and 500) ng/ml were given orally for seven days to experimental animals (mice) to measure the cytigenetic effects of this toxin by employing the following parameters (mitotic index (MI), micronulcleus (MN), chromosomal aberrations (CAs) and sperm abnormalities) in compression with negative control PBS (phosphate buffer slaine).

The effects of the three dosed of ETA on mice indicated that all doses caused significant decrease in MI and significant increase in CAs, MN percentage and increase sperm abnormalities in compression with control and these significant differences are dose degraded.

التخصصات الرئيسية

الأحياء

عدد الصفحات

133

قائمة المحتويات

• APA
• MLA
• AMA

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-600968