Isolation and identification of clinical Pseudomonas aeruginosa producing exotoxin a and studying its toxic effect in mice
Other Title(s)
عزل و تشخيص بكتريا Pseudomonas aeruginosa المرضية المنتجة للسم الخارجي A و دراسة تأثيره السمي في الفئران
Dissertant
Thesis advisor
al-Tai, Maha Fakhri
Hamzah, Subhi Jawad
University
University of Baghdad
Faculty
College of Science
Department
Biotechnology Department
University Country
Iraq
Degree
Master
Degree Date
2012
English Abstract
Forty-five pathogenic bacterial isolates were identified as P.
aeruginosa which isolated from seventy-five patients suffering from various infections, twenty-five isolates were found producing exotoxin A as detected by specific ELISA kit.
Accordingly, three isolates (PA10, PA11 and PA15) were selected according to their productivity and site of infections as detected by ELISA kit.
Exotoxins A were partially purified using 90% saturation of ammonium sulphate then DEAE-cellulose column.
Isolate PA15 was found able to produce 32.08ng/ml of active exotoxin A (ETA) with a protein of about 0.063 mg/ml.
Molecular weight of the toxin was determined to be 65,000 Dalton.
Subsequently, three dose of ETA (125, 250 and 500) ng/ml were given orally for seven days to experimental animals (mice) to measure the cytigenetic effects of this toxin by employing the following parameters (mitotic index (MI), micronulcleus (MN), chromosomal aberrations (CAs) and sperm abnormalities) in compression with negative control PBS (phosphate buffer slaine).
The effects of the three dosed of ETA on mice indicated that all doses caused significant decrease in MI and significant increase in CAs, MN percentage and increase sperm abnormalities in compression with control and these significant differences are dose degraded.
Main Subjects
No. of Pages
133
Table of Contents
Table of contents.
Abstract.
Abstract in Arabic.
Introduction.
Chapter One : Literature review.
Chapter Two : Materials and methods.
Chapter Three : Results and discussions.
Conclusions and recommendations.
References.
American Psychological Association (APA)
Nafi, Sarah Qusayy. (2012). Isolation and identification of clinical Pseudomonas aeruginosa producing exotoxin a and studying its toxic effect in mice. (Master's theses Theses and Dissertations Master). University of Baghdad, Iraq
https://search.emarefa.net/detail/BIM-600968
Modern Language Association (MLA)
Nafi, Sarah Qusayy. Isolation and identification of clinical Pseudomonas aeruginosa producing exotoxin a and studying its toxic effect in mice. (Master's theses Theses and Dissertations Master). University of Baghdad. (2012).
https://search.emarefa.net/detail/BIM-600968
American Medical Association (AMA)
Nafi, Sarah Qusayy. (2012). Isolation and identification of clinical Pseudomonas aeruginosa producing exotoxin a and studying its toxic effect in mice. (Master's theses Theses and Dissertations Master). University of Baghdad, Iraq
https://search.emarefa.net/detail/BIM-600968
Language
English
Data Type
Arab Theses
Record ID
BIM-600968
