Alpha-amanitin poisoning, nephrotoxicity and oxidative stress : an experimental mouse model
المؤلفون المشاركون
Girisgin, Sadiq Abd Allah
Dundar, Zerrin Defne
Kilinc, Ibrahim
Colak, Tamir
Oltulu, Pembe
Ergin, Muhammad
المصدر
Iranian Red Crescent Medical Journal
العدد
المجلد 17، العدد 8 (31 أغسطس/آب 2015)، ص ص. 1-8، 8ص.
الناشر
تاريخ النشر
2015-08-31
دولة النشر
الإمارات العربية المتحدة
عدد الصفحات
8
التخصصات الرئيسية
الموضوعات
الملخص EN
Background : Alpha-amanitin (α-AMA) plays a major role in Amanita phalloides poisoning, showing toxic effects on multi-organs, particularly on the liver and kidneys.
Studies have shown a relationship between α-AMA-related injuries and reactive oxygen species.
Objectives : We aimed to investigate whether there is renal injury and its relationship with oxidative stress after intraperitoneal injection of α-AMA in mice experimental poisoning models.
Materials and Methods : There were 37 male BALB/c laboratory mice treated with α-AMA, according to the study groups: control group (n = 7); low dose (0.2 mg/kg) (n = 10); moderate dose (0.6 mg/kg) (n = 10), and high dose (1 mg/kg) (n = 10).
The sample size was detected according to the ethical committee’s decision as well as similar studies in the literature.
After a 48-hour follow-up period, all the subjects were sacrificed for pathological and biochemical assays.
The study was held in Turkey.
Results: α-AMA poisoning in mice results in inflammatory changes and necrosis in renal structures.
There were statistically significant differences between the study groups regarding measured levels of catalase, superoxide dismutase, glutathione peroxidase, total antioxidant status (TAS), total oxidant status (TOS) and malonyl dialdehyde in renal homogenates of mice (P < 0.001, P < 0.001, P < 0.001, P < 0.001, P < 0.001, and P = 0.001, respectively).
The TOS and TAS measurements helped to eliminate cumbersome analysis of diverse oxidant and antioxidant molecules.
The TOS levels in renal homogenate of mice were significantly higher in all the intoxication groups compared to the control group (5.73, 7.02, 7.77, and 9.65 mmol trolox eq/g protein and P = 0.002, P = 0.001, and P = 0.001, respectively).
The TAS levels in moderate and high-dose groups were significantly lower than all the other groups treated with α-AMA (0.130, 0.152, 0.065, and 0.087 mmol trolox eq/g protein and P = 0.031, P = 0.001, and P = 0.001, respectively).
Conclusions : Our results indicated that α-AMA poisoning in mice led to inflammatory changes and necrosis in renal structures.
Biochemical analysis showed a shift in the oxidative/anti-oxidative balance towards the oxidative status.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ergin, Muhammad& Dundar, Zerrin Defne& Kilinc, Ibrahim& Colak, Tamir& Oltulu, Pembe& Girisgin, Sadiq Abd Allah. 2015. Alpha-amanitin poisoning, nephrotoxicity and oxidative stress : an experimental mouse model. Iranian Red Crescent Medical Journal،Vol. 17, no. 8, pp.1-8.
https://search.emarefa.net/detail/BIM-606001
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ergin, Muhammad…[et al.]. Alpha-amanitin poisoning, nephrotoxicity and oxidative stress : an experimental mouse model. Iranian Red Crescent Medical Journal Vol. 17, no. 8 (Aug. 2015), pp.1-8.
https://search.emarefa.net/detail/BIM-606001
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ergin, Muhammad& Dundar, Zerrin Defne& Kilinc, Ibrahim& Colak, Tamir& Oltulu, Pembe& Girisgin, Sadiq Abd Allah. Alpha-amanitin poisoning, nephrotoxicity and oxidative stress : an experimental mouse model. Iranian Red Crescent Medical Journal. 2015. Vol. 17, no. 8, pp.1-8.
https://search.emarefa.net/detail/BIM-606001
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 7-8
رقم السجل
BIM-606001
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر