Alpha-amanitin poisoning, nephrotoxicity and oxidative stress : an experimental mouse model
Joint Authors
Girisgin, Sadiq Abd Allah
Dundar, Zerrin Defne
Kilinc, Ibrahim
Colak, Tamir
Oltulu, Pembe
Ergin, Muhammad
Source
Iranian Red Crescent Medical Journal
Issue
Vol. 17, Issue 8 (31 Aug. 2015), pp.1-8, 8 p.
Publisher
Publication Date
2015-08-31
Country of Publication
United Arab Emirates
No. of Pages
8
Main Subjects
Topics
Abstract EN
Background : Alpha-amanitin (α-AMA) plays a major role in Amanita phalloides poisoning, showing toxic effects on multi-organs, particularly on the liver and kidneys.
Studies have shown a relationship between α-AMA-related injuries and reactive oxygen species.
Objectives : We aimed to investigate whether there is renal injury and its relationship with oxidative stress after intraperitoneal injection of α-AMA in mice experimental poisoning models.
Materials and Methods : There were 37 male BALB/c laboratory mice treated with α-AMA, according to the study groups: control group (n = 7); low dose (0.2 mg/kg) (n = 10); moderate dose (0.6 mg/kg) (n = 10), and high dose (1 mg/kg) (n = 10).
The sample size was detected according to the ethical committee’s decision as well as similar studies in the literature.
After a 48-hour follow-up period, all the subjects were sacrificed for pathological and biochemical assays.
The study was held in Turkey.
Results: α-AMA poisoning in mice results in inflammatory changes and necrosis in renal structures.
There were statistically significant differences between the study groups regarding measured levels of catalase, superoxide dismutase, glutathione peroxidase, total antioxidant status (TAS), total oxidant status (TOS) and malonyl dialdehyde in renal homogenates of mice (P < 0.001, P < 0.001, P < 0.001, P < 0.001, P < 0.001, and P = 0.001, respectively).
The TOS and TAS measurements helped to eliminate cumbersome analysis of diverse oxidant and antioxidant molecules.
The TOS levels in renal homogenate of mice were significantly higher in all the intoxication groups compared to the control group (5.73, 7.02, 7.77, and 9.65 mmol trolox eq/g protein and P = 0.002, P = 0.001, and P = 0.001, respectively).
The TAS levels in moderate and high-dose groups were significantly lower than all the other groups treated with α-AMA (0.130, 0.152, 0.065, and 0.087 mmol trolox eq/g protein and P = 0.031, P = 0.001, and P = 0.001, respectively).
Conclusions : Our results indicated that α-AMA poisoning in mice led to inflammatory changes and necrosis in renal structures.
Biochemical analysis showed a shift in the oxidative/anti-oxidative balance towards the oxidative status.
American Psychological Association (APA)
Ergin, Muhammad& Dundar, Zerrin Defne& Kilinc, Ibrahim& Colak, Tamir& Oltulu, Pembe& Girisgin, Sadiq Abd Allah. 2015. Alpha-amanitin poisoning, nephrotoxicity and oxidative stress : an experimental mouse model. Iranian Red Crescent Medical Journal،Vol. 17, no. 8, pp.1-8.
https://search.emarefa.net/detail/BIM-606001
Modern Language Association (MLA)
Ergin, Muhammad…[et al.]. Alpha-amanitin poisoning, nephrotoxicity and oxidative stress : an experimental mouse model. Iranian Red Crescent Medical Journal Vol. 17, no. 8 (Aug. 2015), pp.1-8.
https://search.emarefa.net/detail/BIM-606001
American Medical Association (AMA)
Ergin, Muhammad& Dundar, Zerrin Defne& Kilinc, Ibrahim& Colak, Tamir& Oltulu, Pembe& Girisgin, Sadiq Abd Allah. Alpha-amanitin poisoning, nephrotoxicity and oxidative stress : an experimental mouse model. Iranian Red Crescent Medical Journal. 2015. Vol. 17, no. 8, pp.1-8.
https://search.emarefa.net/detail/BIM-606001
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 7-8
Record ID
BIM-606001