Trials for propagation of egg drop syndrome (EDS-76)‎ virus on different tissue culture types

العناوين الأخرى

محاولات لتمرير الفيروس المسبب لظاهرة تدنى البيض في أنواع مختلفة من خلايا الزرع النسيجي

المؤلفون المشاركون

Rufail, Nansi Butrus
Ibrahim, Nadiyah Muhammad
al-Burdini, Fikryah Abd al-Hafiz
al-Makaki, Halah Muhammad

المصدر

Kafr El-Sheikh Veterinary Medical Journal

الناشر

جامعة كفر الشيخ كلية الطب البيطري

تاريخ النشر

2003-04-30

دولة النشر

مصر

عدد الصفحات

10

التخصصات الرئيسية

الطب البيطري

الملخص الإنجليزي

Three types of cell cultures, chicken embryo fibroblast (CEF), chicken kidney cells (CK) and chicken liver cells (CL) were prepared for adaptation and propagation from duck embryo adapted strain D76 of egg drop syndrome (EDS).

After five passages, the virus titre reached 109, 108 and 107 TCID50/0.1 ml in CEF, CK and CL, respectively, where the cytopathic effect of the virus began to appear at 3, 3 and 4 days post inoculation, respectively.

The studies of the virus growth curve revealed that the peak titre was detected at 72, 80 and 92 hours post inoculation in CEF, CK and CL, respectively.

Therefore, the use of cell culture system instead of embryonated duck egg system for adaptation, propagation and titration of EDS-76 is recommended because it is rapid, sensitive, specific and reduce the chance for contamination.

نوع البيانات

أوراق مؤتمرات

رقم السجل

BIM-628508

• APA
• MLA
• AMA