Association of t (14 ; 18)‎ chromosomal translocation to hodgkin’s lymphoma in Iraqi patients using multiplex PCR technique

الملخص EN

Translocation t (14 ; 18) (q32 ; q21) is a common cytogenetic abnormality in B- cell lymphoma, especially in non-Hodgkin’s lymphoma, but it is rarely seen in Hodgkin’s lymphoma (HL).

In the current study, t(14 ; 18) chromosomal translocation was detected in Hodgkin’s lymphoma of Iraqi patients by multiplex PCR with multiple primers and direct sequence by Sanger method with Agencourt® CleanSEQ® dye-terminator Removal Kit.

DNA was extracted from 50 samples of paraffin- embedded tissue, 25 samples of blood by using QIAamp DNA Mini Kit (Qiagen / Germany) and 10 samples from reactive hyperplasia as control.

The t (14, 18) was detected in 26(35 %) cases that showed IGH/BCL2 gene rearrangement with a significant difference compared to control (P<0.01) or hyperplasia.

The detected breakpoints represent as 5(19.2 %) for major breakpoint MBR1, 4(15.38 %) for MBR2 and 3(11.5 %); 2(8 %); 7(27 %) for 3ʹMBR1, 3ʹMBR2 and 3ʹMBR4 respectively.

Break points of MCR1, MCR2, 5ʹMCR were detected as 4(15.38 %); 1(4 %) and 2(8 %)) respectively.

On the other hand, no breakpoint was detected for 3ʹMBR3.

Thus, this study reported an increased frequency of breakpoints in MBR regions in HL patients than mcr cluster and an increase in t (14 ; 18) translocation in adult’s age and in male gander.

In conclusion t (14 ;18)(q32 ; q21) was detected in B- cell lymphoma by multiple primers for all three breakpoint regions, the MBR, 3ʹ MBR and mcr with multiplex PCR amplification.