Association of t (14 ; 18) chromosomal translocation to hodgkin’s lymphoma in Iraqi patients using multiplex PCR technique
Joint Authors
al-Faysal, Abd al-Husayn Muwayt
Tubal, Khalid
Hamzah, Isra Husayn
al-Rikabi, Abd al-Amir Nasir Ghalub
Source
Iraqi Journal of Biotechnology
Issue
Vol. 14, Issue 2 (31 Dec. 2015), pp.80-90, 11 p.
Publisher
University of Baghdad Genetic Engineering and Biotechnology for Postgraduate Studies
Publication Date
2015-12-31
Country of Publication
Iraq
No. of Pages
11
Main Subjects
Abstract EN
Translocation t (14 ; 18) (q32 ; q21) is a common cytogenetic abnormality in B- cell lymphoma, especially in non-Hodgkin’s lymphoma, but it is rarely seen in Hodgkin’s lymphoma (HL).
In the current study, t(14 ; 18) chromosomal translocation was detected in Hodgkin’s lymphoma of Iraqi patients by multiplex PCR with multiple primers and direct sequence by Sanger method with Agencourt® CleanSEQ® dye-terminator Removal Kit.
DNA was extracted from 50 samples of paraffin- embedded tissue, 25 samples of blood by using QIAamp DNA Mini Kit (Qiagen / Germany) and 10 samples from reactive hyperplasia as control.
The t (14, 18) was detected in 26(35 %) cases that showed IGH/BCL2 gene rearrangement with a significant difference compared to control (P<0.01) or hyperplasia.
The detected breakpoints represent as 5(19.2 %) for major breakpoint MBR1, 4(15.38 %) for MBR2 and 3(11.5 %); 2(8 %); 7(27 %) for 3ʹMBR1, 3ʹMBR2 and 3ʹMBR4 respectively.
Break points of MCR1, MCR2, 5ʹMCR were detected as 4(15.38 %); 1(4 %) and 2(8 %)) respectively.
On the other hand, no breakpoint was detected for 3ʹMBR3.
Thus, this study reported an increased frequency of breakpoints in MBR regions in HL patients than mcr cluster and an increase in t (14 ; 18) translocation in adult’s age and in male gander.
In conclusion t (14 ;18)(q32 ; q21) was detected in B- cell lymphoma by multiple primers for all three breakpoint regions, the MBR, 3ʹ MBR and mcr with multiplex PCR amplification.
American Psychological Association (APA)
Hamzah, Isra Husayn& al-Faysal, Abd al-Husayn Muwayt& al-Rikabi, Abd al-Amir Nasir Ghalub& Tubal, Khalid. 2015. Association of t (14 ; 18) chromosomal translocation to hodgkin’s lymphoma in Iraqi patients using multiplex PCR technique. Iraqi Journal of Biotechnology،Vol. 14, no. 2, pp.80-90.
https://search.emarefa.net/detail/BIM-644787
Modern Language Association (MLA)
Hamzah, Isra Husayn…[et al.]. Association of t (14 ; 18) chromosomal translocation to hodgkin’s lymphoma in Iraqi patients using multiplex PCR technique. Iraqi Journal of Biotechnology Vol. 14, no. 2 (2015), pp.80-90.
https://search.emarefa.net/detail/BIM-644787
American Medical Association (AMA)
Hamzah, Isra Husayn& al-Faysal, Abd al-Husayn Muwayt& al-Rikabi, Abd al-Amir Nasir Ghalub& Tubal, Khalid. Association of t (14 ; 18) chromosomal translocation to hodgkin’s lymphoma in Iraqi patients using multiplex PCR technique. Iraqi Journal of Biotechnology. 2015. Vol. 14, no. 2, pp.80-90.
https://search.emarefa.net/detail/BIM-644787
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 89-90
Record ID
BIM-644787