Using 2,4-dinitrotoluene as growth substrate by bacterial isolate : effect of some growth conditions

العناوين الأخرى

استخدام DNT-2,4 كعامل نمو لعزلات البكتيريا و إيجاد الظروف المثلى لها

مقدم أطروحة جامعية

Khulayfat, Muayyad S.

مشرف أطروحة جامعية

Khulayfat, Khalid Muhammad

أعضاء اللجنة

Abu Darwish, Muhammad Sanad
al-Shurafa, Khalid Yusuf Ahmad
al-Zaraini, Wail Ali

الجامعة

جامعة مؤتة

الكلية

كلية العلوم

القسم الأكاديمي

قسم الأحياء

دولة الجامعة

الأردن

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2015

الملخص الإنجليزي

A total six different bacteria were isolated from two years TNT contaminated soil samples collected from ma`an quarries , south of Jordan.

TNT is a nitroaromatic compound which have as a intermediates product 2,4-DNT and 2,6-DNT mixtures ,this mixture used in the manufacture of dyes, pesticides, polymers and explosives .

Typically manufacturing of TNT produced 76 % 2,4-DNT , 19 % 2,6 DNT ( ratio 4 : 1 ) and small amount of other compound .

This study to find and optimized bacteria that have the ability to degrade 2,4-DNT.

Many methods used to identified bacteria starting with colony morphology, gram stain, oxidase test and 16s ribosomal ( rRNA ) gene sequencing.

This bacteria cultured in BSM that have only 2,4-DNT as a carbon , nitrogen source and sole energy, were O.D.

measured at 600 nm and 418 nm every 8 hours , numerous condition studied pH, Temperature and carbon source, nitrogen source .

Two isolated bacteria show the ability to degrade 2,4-DNT , this bacteria identified by 16S rRNA as Bacillus sp.

SP79 and Bacillus lichenformis, the degradation rate was Bacillus Sp.

SP79 0.35 ( μM / h ) and Bacillus licheniformis 0.67 ( μM / h ) , growth and degradation ability show small effect by physical condition ( pH, Temperature ) and highly effect by chemical condition ( carbon and nitrogen source ) according to what bacteria prefer .

التخصصات الرئيسية

الأحياء

الموضوعات

عدد الصفحات

58

قائمة المحتويات

Table of contents.

Abstract.

Abstract in Arabic.

Chapter One : Theoretical background.

Chapter Two : Review of literature.

Chapter Three : Design and methodology.

Chapter Four : Findings and discussions and recommendation.

References.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Khulayfat, Muayyad S.. (2015). Using 2,4-dinitrotoluene as growth substrate by bacterial isolate : effect of some growth conditions. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-656457

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Khulayfat, Muayyad S.. Using 2,4-dinitrotoluene as growth substrate by bacterial isolate : effect of some growth conditions. (Master's theses Theses and Dissertations Master). Mutah University. (2015).
https://search.emarefa.net/detail/BIM-656457

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Khulayfat, Muayyad S.. (2015). Using 2,4-dinitrotoluene as growth substrate by bacterial isolate : effect of some growth conditions. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-656457

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-656457