Using 2,4-dinitrotoluene as growth substrate by bacterial isolate : effect of some growth conditions
Other Title(s)
استخدام DNT-2,4 كعامل نمو لعزلات البكتيريا و إيجاد الظروف المثلى لها
Dissertant
Thesis advisor
Comitee Members
Abu Darwish, Muhammad Sanad
al-Shurafa, Khalid Yusuf Ahmad
al-Zaraini, Wail Ali
University
Mutah University
Faculty
Faculty of Science
Department
Department of Biological Sciences
University Country
Jordan
Degree
Master
Degree Date
2015
English Abstract
A total six different bacteria were isolated from two years TNT contaminated soil samples collected from ma`an quarries , south of Jordan.
TNT is a nitroaromatic compound which have as a intermediates product 2,4-DNT and 2,6-DNT mixtures ,this mixture used in the manufacture of dyes, pesticides, polymers and explosives .
Typically manufacturing of TNT produced 76 % 2,4-DNT , 19 % 2,6 DNT ( ratio 4 : 1 ) and small amount of other compound .
This study to find and optimized bacteria that have the ability to degrade 2,4-DNT.
Many methods used to identified bacteria starting with colony morphology, gram stain, oxidase test and 16s ribosomal ( rRNA ) gene sequencing.
This bacteria cultured in BSM that have only 2,4-DNT as a carbon , nitrogen source and sole energy, were O.D.
measured at 600 nm and 418 nm every 8 hours , numerous condition studied pH, Temperature and carbon source, nitrogen source .
Two isolated bacteria show the ability to degrade 2,4-DNT , this bacteria identified by 16S rRNA as Bacillus sp.
SP79 and Bacillus lichenformis, the degradation rate was Bacillus Sp.
SP79 0.35 ( μM / h ) and Bacillus licheniformis 0.67 ( μM / h ) , growth and degradation ability show small effect by physical condition ( pH, Temperature ) and highly effect by chemical condition ( carbon and nitrogen source ) according to what bacteria prefer .
Main Subjects
Topics
No. of Pages
58
Table of Contents
Table of contents.
Abstract.
Abstract in Arabic.
Chapter One : Theoretical background.
Chapter Two : Review of literature.
Chapter Three : Design and methodology.
Chapter Four : Findings and discussions and recommendation.
References.
American Psychological Association (APA)
Khulayfat, Muayyad S.. (2015). Using 2,4-dinitrotoluene as growth substrate by bacterial isolate : effect of some growth conditions. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-656457
Modern Language Association (MLA)
Khulayfat, Muayyad S.. Using 2,4-dinitrotoluene as growth substrate by bacterial isolate : effect of some growth conditions. (Master's theses Theses and Dissertations Master). Mutah University. (2015).
https://search.emarefa.net/detail/BIM-656457
American Medical Association (AMA)
Khulayfat, Muayyad S.. (2015). Using 2,4-dinitrotoluene as growth substrate by bacterial isolate : effect of some growth conditions. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-656457
Language
English
Data Type
Arab Theses
Record ID
BIM-656457
