Optimization of the biodegradation of 2,4- dinitrotoluene compound (2,4-DNT)‎ by bacteria isolated from quarries in Jordan

العناوين الأخرى

الظروف المعيارية لتحطيم مركب 2,4 دي نيترو تولوين بواسطة بكتيريا عزلت من تربة مقلع موجود في الأردن

مقدم أطروحة جامعية

al-Shuaybat, Said Hashim

مشرف أطروحة جامعية

Khulayfat, Khalid Muhammad

أعضاء اللجنة

Abu Darwish, Muhammad Sanad
al-Shurafa, Khalid Yusuf Ahmad
al-Zaraini, Wail Ali

الجامعة

جامعة مؤتة

الكلية

كلية العلوم

القسم الأكاديمي

قسم الأحياء

دولة الجامعة

الأردن

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2015

الملخص الإنجليزي

Two bacteria was isolated from quarries' soil contaminated with 2,4-DNT compound for 5 years ago, from Ma'an city southern of Jordan.

It was identified by using morphological and biochemical characteristics as Acinetobacter calcoaceticus , Acinetobacter sp.

Where found to use the 2,4-DNT compound ( is an Nitroaromatic organic compound with the formula C7H6N2O4 ,which is an important intermediate in the explosive industries and considered as a U.S.

Environmental Protection Agency priority pollutant ) as alternative carbon and nitrogen source at 110 μM concentration .

degradation ability was monitored through the disappearance of the substrate and the growth of bacterial cells on the substrate.

The degradation is a catabolic process catalyzed by enzyme activity .

A dioxygenase oxidizes the nitro group either at ortho or meta position.

The degrading ability tested by using base salt media (BSM) were done in 500 ml Erlenmeyer flasks with working volume of 100 ml on a rotary shaker (37°C, 150 rpm) For 72 hour .

The best results of degradation of 2,4-DNT compound were obtained with 110μM substrate concentration ,40oC , PH 7.5 , 8 .

The carbon sources which used at ( 0.2 %) enhanced the growth and affected the biodegradation differently , glucose reduce the degradation percentage .

Nitrogen sources used enhanced the growth for 8 folds of that control ,also enhanced the biodegradation rates of 2,4-DNT at different levels .

التخصصات الرئيسية

الأحياء

الموضوعات

عدد الصفحات

66

قائمة المحتويات

Table of contents.

Abstract.

Abstract in Arabic.

Chapter One : Theoretical background.

Chapter Two : Literature review.

Chapter Three : Design and methodology.

Chapter Four : Results, discussions and recommendations.

References.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

al-Shuaybat, Said Hashim. (2015). Optimization of the biodegradation of 2,4- dinitrotoluene compound (2,4-DNT) by bacteria isolated from quarries in Jordan. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-656483

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

al-Shuaybat, Said Hashim. Optimization of the biodegradation of 2,4- dinitrotoluene compound (2,4-DNT) by bacteria isolated from quarries in Jordan. (Master's theses Theses and Dissertations Master). Mutah University. (2015).
https://search.emarefa.net/detail/BIM-656483

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

al-Shuaybat, Said Hashim. (2015). Optimization of the biodegradation of 2,4- dinitrotoluene compound (2,4-DNT) by bacteria isolated from quarries in Jordan. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-656483

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-656483