Optimization of the biodegradation of 2,4- dinitrotoluene compound (2,4-DNT) by bacteria isolated from quarries in Jordan
Other Title(s)
الظروف المعيارية لتحطيم مركب 2,4 دي نيترو تولوين بواسطة بكتيريا عزلت من تربة مقلع موجود في الأردن
Dissertant
Thesis advisor
Comitee Members
Abu Darwish, Muhammad Sanad
al-Shurafa, Khalid Yusuf Ahmad
al-Zaraini, Wail Ali
University
Mutah University
Faculty
Faculty of Science
Department
Department of Biological Sciences
University Country
Jordan
Degree
Master
Degree Date
2015
English Abstract
Two bacteria was isolated from quarries' soil contaminated with 2,4-DNT compound for 5 years ago, from Ma'an city southern of Jordan.
It was identified by using morphological and biochemical characteristics as Acinetobacter calcoaceticus , Acinetobacter sp.
Where found to use the 2,4-DNT compound ( is an Nitroaromatic organic compound with the formula C7H6N2O4 ,which is an important intermediate in the explosive industries and considered as a U.S.
Environmental Protection Agency priority pollutant ) as alternative carbon and nitrogen source at 110 μM concentration .
degradation ability was monitored through the disappearance of the substrate and the growth of bacterial cells on the substrate.
The degradation is a catabolic process catalyzed by enzyme activity .
A dioxygenase oxidizes the nitro group either at ortho or meta position.
The degrading ability tested by using base salt media (BSM) were done in 500 ml Erlenmeyer flasks with working volume of 100 ml on a rotary shaker (37°C, 150 rpm) For 72 hour .
The best results of degradation of 2,4-DNT compound were obtained with 110μM substrate concentration ,40oC , PH 7.5 , 8 .
The carbon sources which used at ( 0.2 %) enhanced the growth and affected the biodegradation differently , glucose reduce the degradation percentage .
Nitrogen sources used enhanced the growth for 8 folds of that control ,also enhanced the biodegradation rates of 2,4-DNT at different levels .
Main Subjects
Topics
No. of Pages
66
Table of Contents
Table of contents.
Abstract.
Abstract in Arabic.
Chapter One : Theoretical background.
Chapter Two : Literature review.
Chapter Three : Design and methodology.
Chapter Four : Results, discussions and recommendations.
References.
American Psychological Association (APA)
al-Shuaybat, Said Hashim. (2015). Optimization of the biodegradation of 2,4- dinitrotoluene compound (2,4-DNT) by bacteria isolated from quarries in Jordan. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-656483
Modern Language Association (MLA)
al-Shuaybat, Said Hashim. Optimization of the biodegradation of 2,4- dinitrotoluene compound (2,4-DNT) by bacteria isolated from quarries in Jordan. (Master's theses Theses and Dissertations Master). Mutah University. (2015).
https://search.emarefa.net/detail/BIM-656483
American Medical Association (AMA)
al-Shuaybat, Said Hashim. (2015). Optimization of the biodegradation of 2,4- dinitrotoluene compound (2,4-DNT) by bacteria isolated from quarries in Jordan. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-656483
Language
English
Data Type
Arab Theses
Record ID
BIM-656483
