Comparison of a genus-specific conventional PCR and a species-specific nested-PCR for malaria diagnosis using FTA collected samples from Kingdom of Saudi Arabia
المؤلف
المصدر
Journal of the Egyptian Society of Parasitology
العدد
المجلد 45، العدد 3 (31 ديسمبر/كانون الأول 2015)، ص ص. 457-466، 10ص.
الناشر
الجمعية المصرية لعلم الطفيليات
تاريخ النشر
2015-12-31
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الموضوعات
الملخص EN
Molecular tools are increasingly accepted as the most sensitive and reliable techniques for malaria diagnosis and epidemiological surveys.
Also, collection of finger prick blood spots onto filter papers is the most simple and affordable method for samples preservation and posterior molecular analysis, especially in rural endemic regions where malaria remains a major health problem.
Two malaria molecular diagnostic tests, a Plasmodium genus-specific conventional PCR and a Plasmodium species-specific Nested PCR, were evaluated using DNA templates prepared from Whatman- FTA®-cards' dry blood spots using both, Methanol-fixation/Heat-extraction and FTA® commercial purification kit.
A total of 121 blood sam-ples were collected from six Saudi south-western endemic districts both, as thick and thin films for routine microscopic screening and onto FTA®- cards for molecular studies.
Out of the 121 samples, 75 were P.
falciparum positive by at least one technique.
No other species of Plasmodium were detected.
P.
falciparum parasites were identified in 69/75 (92%) samples by microscopic screening in health care centers.
P.
genus-specific PCR was able to amplify P.
falciparum DNA in 41/75 (55%) and 59/75 (79%) samples using Methanol-fixation/Heat-extraction and FTA® purification kit, respectively.
P.
species-specific Nested PCR revealed 68/75 (91%) and 75/75 (100%) positive samples using DNA templates were isolated by Methanol-fixation/Heatextraction and FTA® purification methods, respectively.
The species-specific Nested PCR applied to Whatman-FTA® preserved and processed blood samples represents the best alternative to classical microscopy for malaria diagnosis, particularly in epidemiological screening.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Harithi, Said A.. 2015. Comparison of a genus-specific conventional PCR and a species-specific nested-PCR for malaria diagnosis using FTA collected samples from Kingdom of Saudi Arabia. Journal of the Egyptian Society of Parasitology،Vol. 45, no. 3, pp.457-466.
https://search.emarefa.net/detail/BIM-668433
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Harithi, Said A.. Comparison of a genus-specific conventional PCR and a species-specific nested-PCR for malaria diagnosis using FTA collected samples from Kingdom of Saudi Arabia. Journal of the Egyptian Society of Parasitology Vol. 45, no. 3 (Dec. 2015), pp.457-466.
https://search.emarefa.net/detail/BIM-668433
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Harithi, Said A.. Comparison of a genus-specific conventional PCR and a species-specific nested-PCR for malaria diagnosis using FTA collected samples from Kingdom of Saudi Arabia. Journal of the Egyptian Society of Parasitology. 2015. Vol. 45, no. 3, pp.457-466.
https://search.emarefa.net/detail/BIM-668433
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 464-466
رقم السجل
BIM-668433
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر