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Comparison of a genus-specific conventional PCR and a species-specific nested-PCR for malaria diagnosis using FTA collected samples from Kingdom of Saudi Arabia
Author
Source
Journal of the Egyptian Society of Parasitology
Issue
Vol. 45, Issue 3 (31 Dec. 2015), pp.457-466, 10 p.
Publisher
The Egyptian Society of Parasitology
Publication Date
2015-12-31
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Topics
Abstract EN
Molecular tools are increasingly accepted as the most sensitive and reliable techniques for malaria diagnosis and epidemiological surveys.
Also, collection of finger prick blood spots onto filter papers is the most simple and affordable method for samples preservation and posterior molecular analysis, especially in rural endemic regions where malaria remains a major health problem.
Two malaria molecular diagnostic tests, a Plasmodium genus-specific conventional PCR and a Plasmodium species-specific Nested PCR, were evaluated using DNA templates prepared from Whatman- FTA®-cards' dry blood spots using both, Methanol-fixation/Heat-extraction and FTA® commercial purification kit.
A total of 121 blood sam-ples were collected from six Saudi south-western endemic districts both, as thick and thin films for routine microscopic screening and onto FTA®- cards for molecular studies.
Out of the 121 samples, 75 were P.
falciparum positive by at least one technique.
No other species of Plasmodium were detected.
P.
falciparum parasites were identified in 69/75 (92%) samples by microscopic screening in health care centers.
P.
genus-specific PCR was able to amplify P.
falciparum DNA in 41/75 (55%) and 59/75 (79%) samples using Methanol-fixation/Heat-extraction and FTA® purification kit, respectively.
P.
species-specific Nested PCR revealed 68/75 (91%) and 75/75 (100%) positive samples using DNA templates were isolated by Methanol-fixation/Heatextraction and FTA® purification methods, respectively.
The species-specific Nested PCR applied to Whatman-FTA® preserved and processed blood samples represents the best alternative to classical microscopy for malaria diagnosis, particularly in epidemiological screening.
American Psychological Association (APA)
al-Harithi, Said A.. 2015. Comparison of a genus-specific conventional PCR and a species-specific nested-PCR for malaria diagnosis using FTA collected samples from Kingdom of Saudi Arabia. Journal of the Egyptian Society of Parasitology،Vol. 45, no. 3, pp.457-466.
https://search.emarefa.net/detail/BIM-668433
Modern Language Association (MLA)
al-Harithi, Said A.. Comparison of a genus-specific conventional PCR and a species-specific nested-PCR for malaria diagnosis using FTA collected samples from Kingdom of Saudi Arabia. Journal of the Egyptian Society of Parasitology Vol. 45, no. 3 (Dec. 2015), pp.457-466.
https://search.emarefa.net/detail/BIM-668433
American Medical Association (AMA)
al-Harithi, Said A.. Comparison of a genus-specific conventional PCR and a species-specific nested-PCR for malaria diagnosis using FTA collected samples from Kingdom of Saudi Arabia. Journal of the Egyptian Society of Parasitology. 2015. Vol. 45, no. 3, pp.457-466.
https://search.emarefa.net/detail/BIM-668433
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 464-466
Record ID
BIM-668433