Sirtuin 1 gene rs2273773 C > T single nucleotide polymorphism and protein oxidation markers in asthmatic patients

الملخص EN

Background : Sirtuin-1 (SIRT-1), a protein has been found to protect the cells against oxidative stress due to its deacetylase activity.

In this investigation, we aimed to study SIRT-1 gene rs2273773 C >T single nucleotide polymorphism and markers of serum protein oxidation (protein carbonyl and sulfhydryl groups) in asthmatic patients.

Subjects and methods : 120 asthmatic patients and 120 healthy controls were genotyped for SIRT- 1 gene rs2273773 C > T SNP using polymerase chain reaction – confronting two pair primer method (PCR-CTPP).

Serum protein carbonyl and sulfhydryl groups were measured using colorimetric methods.

Results : SIRT-1 gene rs2273773 C> T SNP genotyping revealed that the TT genotype was significantly higher in the patients compared to the controls (P < 0.05), while there were no significant differences regarding the genotypes TC and CC between the patients and the controls (P> 0.05).

T allele was significantly higher in the patients compared to the controls (P =0.017).

The distribution of the genotypes didn’t differ among the atopic and the non-atopic asthmatic patients, also no difference was found in the genotype distribution according to the severity of asthma (P >0.05).

Serum protein carbonyl group concentration was significantly higher in the patients compared to the controls (P < 0.

001), while serum protein sulfhydryl group content decreased significantly in the patients compared to the controls (P < 0.0001).

No differences in markers of protein oxidation according to SIRT-1 gene rs2273773 C > T genotype were found.

Conclusion: SIRT-1 gene rs2273773 C> T SNP was associated with asthma but not with protein oxidation markers in Egyptian population.

2016 The Authors.

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