Sirtuin 1 gene rs2273773 C > T single nucleotide polymorphism and protein oxidation markers in asthmatic patients

Abstract EN

Background : Sirtuin-1 (SIRT-1), a protein has been found to protect the cells against oxidative stress due to its deacetylase activity.

In this investigation, we aimed to study SIRT-1 gene rs2273773 C >T single nucleotide polymorphism and markers of serum protein oxidation (protein carbonyl and sulfhydryl groups) in asthmatic patients.

Subjects and methods : 120 asthmatic patients and 120 healthy controls were genotyped for SIRT- 1 gene rs2273773 C > T SNP using polymerase chain reaction – confronting two pair primer method (PCR-CTPP).

Serum protein carbonyl and sulfhydryl groups were measured using colorimetric methods.

Results : SIRT-1 gene rs2273773 C> T SNP genotyping revealed that the TT genotype was significantly higher in the patients compared to the controls (P < 0.05), while there were no significant differences regarding the genotypes TC and CC between the patients and the controls (P> 0.05).

T allele was significantly higher in the patients compared to the controls (P =0.017).

The distribution of the genotypes didn’t differ among the atopic and the non-atopic asthmatic patients, also no difference was found in the genotype distribution according to the severity of asthma (P >0.05).

Serum protein carbonyl group concentration was significantly higher in the patients compared to the controls (P < 0.

001), while serum protein sulfhydryl group content decreased significantly in the patients compared to the controls (P < 0.0001).

No differences in markers of protein oxidation according to SIRT-1 gene rs2273773 C > T genotype were found.

Conclusion: SIRT-1 gene rs2273773 C> T SNP was associated with asthma but not with protein oxidation markers in Egyptian population.

2016 The Authors.

Production and hosting by Elsevier B.V.

on behalf of Ain Shams University.

This is an open access article under theCCBY-NC-NDlicense.