Study of fibrinolytic enzyme (streptokinase)‎ produced from clinical streptococcus pyogenes isolates

الملخص EN

Thirty samples of bacterial isolates were collected from patients of Hilla Teaching Hospital during the period from 1st - 31 st / July – 2014 .

These samples were isolated from upper respiratory tract (pharynx) of both sex patients .

The swabs were cultured immediately on tryptic soya agar and incubated at 37 C for 24 hr .

The isolates were identified according to cultural properties and biochemical testes .

The results showed that these isolates were belonged to Streptococcus pyogenes .

The ability of these isolates to produce streptokinase was studied .

The results exhibited presence of two active SPA1 and SPA2 .

The activity of the produced enzyme was studied by fibrin plate method .

Furthermore the effect of pH at different values and different temperatures (-5,5,25,35 and 55C) were examined .

The results revealed that the optimum pH was 6 , while the optimum temperature was 35C .