Study of fibrinolytic enzyme (streptokinase) produced from clinical streptococcus pyogenes isolates
Joint Authors
Ahmad Abbas A.
Nur Sad Allah N.
Nimah, Zahra Abd
Jar Allah, Iman Muhammad
Source
Journal of Babylon University : Journal of Applied and Pure Sciences
Issue
Vol. 24, Issue 2 (30 Jun. 2016), pp.364-369, 6 p.
Publisher
Publication Date
2016-06-30
Country of Publication
Iraq
No. of Pages
6
Main Subjects
Abstract AR
تم في الدراسة الحالية جمع ثلاثون مسحة من بلعوم مرضى الجهاز التنفسي العلوي في مستشفى الحلة الجراحي للفترة من 1-31 تموز / 2014 و من كلا الجنسين و قدر زرعت المسحات مباشرة و على وسط الصويا الصلب.
شخصت عزلات بكتريا المسبحات القيحية اعتمادا على الصفات الزرعية و الفحوصات البايوكيمياوية.
و قد أظهرت النتائج وجود عزلتين كفوأتين في إنتاج الأنزيم الحال للخثرة الدموية بطريقة طبق الفايبرين، و قد رمز لهما (المسبحية القيحية 1 و 2).
و قد درس تأثير كل من الأس الهيدروجيني و درجات الحرارة المختلفة (- 5 و 5 و 35 و 55 م) على فعالية الأنزيم المنتج، و قد تبين أن أفضل أس هيدروجيني 6، و أفضل درجة حرارة هي 35 م°.
Abstract EN
Thirty samples of bacterial isolates were collected from patients of Hilla Teaching Hospital during the period from 1st - 31 st / July – 2014 .
These samples were isolated from upper respiratory tract (pharynx) of both sex patients .
The swabs were cultured immediately on tryptic soya agar and incubated at 37 C for 24 hr .
The isolates were identified according to cultural properties and biochemical testes .
The results showed that these isolates were belonged to Streptococcus pyogenes .
The ability of these isolates to produce streptokinase was studied .
The results exhibited presence of two active SPA1 and SPA2 .
The activity of the produced enzyme was studied by fibrin plate method .
Furthermore the effect of pH at different values and different temperatures (-5,5,25,35 and 55C) were examined .
The results revealed that the optimum pH was 6 , while the optimum temperature was 35C .
American Psychological Association (APA)
Jar Allah, Iman Muhammad& Ahmad Abbas A.& Nur Sad Allah N.& Nimah, Zahra Abd. 2016. Study of fibrinolytic enzyme (streptokinase) produced from clinical streptococcus pyogenes isolates. Journal of Babylon University : Journal of Applied and Pure Sciences،Vol. 24, no. 2, pp.364-369.
https://search.emarefa.net/detail/BIM-684442
Modern Language Association (MLA)
Jar Allah, Iman Muhammad…[et al.]. Study of fibrinolytic enzyme (streptokinase) produced from clinical streptococcus pyogenes isolates. Journal of Babylon University : Journal of Applied and Pure Sciences Vol. 24, no. 2 (2016), pp.364-369.
https://search.emarefa.net/detail/BIM-684442
American Medical Association (AMA)
Jar Allah, Iman Muhammad& Ahmad Abbas A.& Nur Sad Allah N.& Nimah, Zahra Abd. Study of fibrinolytic enzyme (streptokinase) produced from clinical streptococcus pyogenes isolates. Journal of Babylon University : Journal of Applied and Pure Sciences. 2016. Vol. 24, no. 2, pp.364-369.
https://search.emarefa.net/detail/BIM-684442
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 368-369
Record ID
BIM-684442
