Development of cotton leaf curl virus resistant transgenic cotton using antisense ßC1 gene

الملخص EN

Cotton leaf curl virus (CLCuV) is a serious pathogen causing leaf curl disease and affecting the cotton production in major growing areas.

The transgenic cotton (Gossypium hirsutum cv.

Coker 310) plants were developed by using bC1 gene in antisense orientation gene driven by Cauliflower mosaic virus-35S promoter and nos (nopaline synthase) terminator and mediated by Agrobacterium tumefaciens transformation and somatic embryogenesis system.

Molecular confirmation of the transformants was carried out by polymerase chain reaction (PCR) and Southern blot hybridization.

The developed transgenic and inoculated plants remained symptomless till their growth period.

In conclusion, the plants were observed as resistant to CLCuV.

ª 2014 The Authors.

Production and hosting by Elsevier B.V.

on behalf of King Saud University.

This is an open access article under the CC BY-NC-ND license