Development of cotton leaf curl virus resistant transgenic cotton using antisense ßC1 gene
Joint Authors
Suhrab, Sayyid Sartaj
Abd al-Ilah
Husayn, Azamal
Kamal, Muhammad A.
Bhattacharya, P. S.
Rana, D.
Source
Saudi Journal of Biological Sciences
Issue
Vol. 23, Issue 3 (31 May. 2016), pp.358-362, 5 p.
Publisher
Publication Date
2016-05-31
Country of Publication
Saudi Arabia
No. of Pages
5
Main Subjects
Abstract EN
Cotton leaf curl virus (CLCuV) is a serious pathogen causing leaf curl disease and affecting the cotton production in major growing areas.
The transgenic cotton (Gossypium hirsutum cv.
Coker 310) plants were developed by using bC1 gene in antisense orientation gene driven by Cauliflower mosaic virus-35S promoter and nos (nopaline synthase) terminator and mediated by Agrobacterium tumefaciens transformation and somatic embryogenesis system.
Molecular confirmation of the transformants was carried out by polymerase chain reaction (PCR) and Southern blot hybridization.
The developed transgenic and inoculated plants remained symptomless till their growth period.
In conclusion, the plants were observed as resistant to CLCuV.
ª 2014 The Authors.
Production and hosting by Elsevier B.V.
on behalf of King Saud University.
This is an open access article under the CC BY-NC-ND license
American Psychological Association (APA)
Suhrab, Sayyid Sartaj& Kamal, Muhammad A.& Abd al-Ilah& Husayn, Azamal& Bhattacharya, P. S.& Rana, D.. 2016. Development of cotton leaf curl virus resistant transgenic cotton using antisense ßC1 gene. Saudi Journal of Biological Sciences،Vol. 23, no. 3, pp.358-362.
https://search.emarefa.net/detail/BIM-689356
Modern Language Association (MLA)
Suhrab, Sayyid Sartaj…[et al.]. Development of cotton leaf curl virus resistant transgenic cotton using antisense ßC1 gene. Saudi Journal of Biological Sciences Vol. 23, no. 3 (May. 2016), pp.358-362.
https://search.emarefa.net/detail/BIM-689356
American Medical Association (AMA)
Suhrab, Sayyid Sartaj& Kamal, Muhammad A.& Abd al-Ilah& Husayn, Azamal& Bhattacharya, P. S.& Rana, D.. Development of cotton leaf curl virus resistant transgenic cotton using antisense ßC1 gene. Saudi Journal of Biological Sciences. 2016. Vol. 23, no. 3, pp.358-362.
https://search.emarefa.net/detail/BIM-689356
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 361-362
Record ID
BIM-689356