Polymorphism between five local chicken in Erbil using RAPD markers
المؤلفون المشاركون
Abd al-Razzaq, Huria Sabir
Sulayman, Nahil Muhammad Ali
المصدر
ZANCO Journal of Pure and Applied Sciences
العدد
المجلد 28، العدد 1 (31 مارس/آذار 2016)، ص ص. 65-69، 5ص.
الناشر
جامعة صلاح الدين قسم النشر العلمي
تاريخ النشر
2016-03-31
دولة النشر
العراق
عدد الصفحات
5
التخصصات الرئيسية
الموضوعات
الملخص EN
A total of 42 chickens representing White, Brown , Black , Black with Brown neck , Bronze, (twenty five male and Seventeen female) Blood samples were collected from the five local chicken populations.
Blood samples from indigenous chicken populations were collected , and stored at –20°C .
Genomic DNA was extracted from 300 μl of blood .DNA purity and concentrations have been measured by Nano Drop® spectrophotometer .The purity of DNA samples ranged from 1.6 up to 1.9.
Random amplification of polymorphic DNA (RAPD-PCR) was done by using 21 primers .
the PCR reaction had a final volume of 25 μl .
The PCR programmer included an initial denaturation step at 94°C for 5 minutes followed by 40 cycles with 94°C for 1 minutes for DNA denaturation, annealing as mentioned with each primer, extension at 72 _C for 1 minutes and final extension at 72 _C for 5 minutes were carried out.
The PCR products were tested with electrophoresis on 2 % agarose .
The amplified pattern was visualized on a UV transi and photographed .The RAPD bands were scored for their presence (1) or absence (0).
The index of similarity , Genetic distance between each two populations was calculated.
Polymorphism of each primer was calculated.
A total of 2264 loci ranging between 110 and 1900 bp were amplified.
The Primer named OPA-13 amplified a large number of DNA segments from all populations (190 bands).
Primer named OPA-15 amplified a lowest number of DNA segments from all populations (54 bands).
The RAPD profiles of the five local chicken populations were compared, The Primer OPQ-O4 has the highest number of polymorphic band were 23 band.
While the Primer OPA-15 possessed the lowest number of polymorphic band were 7 band.
The highest percentage of the Polymorphisms observed in the primer OPQ-11, a 19.77 when compared with other primers in this study, where the lowest percentage of Polymorphisms was in primer of OPA-01 was 9.48..In this study, a high level of genetic Similarity observed among the chickens was found among the ♂Black and ♀Black its 0.813.
a low level of genetic Similarity is 0.278 was found among the ♂Brown and ♀Black
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Abd al-Razzaq, Huria Sabir& Sulayman, Nahil Muhammad Ali. 2016. Polymorphism between five local chicken in Erbil using RAPD markers. ZANCO Journal of Pure and Applied Sciences،Vol. 28, no. 1, pp.65-69.
https://search.emarefa.net/detail/BIM-693480
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Abd al-Razzaq, Huria Sabir& Sulayman, Nahil Muhammad Ali. Polymorphism between five local chicken in Erbil using RAPD markers. ZANCO Journal of Pure and Applied Sciences Vol. 28, no. 1 (2016), pp.65-69.
https://search.emarefa.net/detail/BIM-693480
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Abd al-Razzaq, Huria Sabir& Sulayman, Nahil Muhammad Ali. Polymorphism between five local chicken in Erbil using RAPD markers. ZANCO Journal of Pure and Applied Sciences. 2016. Vol. 28, no. 1, pp.65-69.
https://search.emarefa.net/detail/BIM-693480
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 69
رقم السجل
BIM-693480
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر