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Physiological study for extracted surface layer from aeromonas hydrophila isolated from diarrhea
المؤلفون المشاركون
Abd, Firyal Jamil
al-Nuri, Lubna Adil
Taj al-Din, Wijdan R.
المصدر
Mesopotamia Environment Journal
العدد
المجلد 2، العدد 3 (31 مايو/أيار 2016)، ص ص. 11-26، 16ص.
الناشر
جامعة بابل مركز البحوث و الدراسات البيئية
تاريخ النشر
2016-05-31
دولة النشر
العراق
عدد الصفحات
16
التخصصات الرئيسية
الموضوعات
- علم الأحياء الدقيقة
- الأطفال
- الأحماض العضوية
- الأحماض الأمينية
- المركبات الأمينية
- العراق
- التشخيص
- البكتيريا اللاهوائية
- الإسهال
الملخص EN
A total of 349 stool samples were collected from the patients with diarrhea during the period from December 2014 to May 2015,the results show that there were 11(3.15%) positive isolates of Aeromonas hydrophila, these isolates were identified by cultural method ,biochemical tests and confirmed by API 20E and Vitek 2 system.Tryptose soy agar that contained Congo red stain was used to detect A.hydrophila isolates that produced surface layer protein (S-layer) ,eight (8) isolates was given positive result on this media.
The S-layer protein was extracted by two methods, Sodium Dodecyl Sulphate (SDS 0.05%) solution was given significantly higher protein concentration in compared with LiCl 5M solution.
Then it was precipitated by two methods also, ammonium per sulfate 80% was given significantly higher protein concentration in compared with acetone Con.95%.
The molecular weight of S-layer protein was determined by SDS-PAGE and the result reveal that it was 52 KDa.
The physiological factors that affecting on the production of S-layer protein from A.hydrophila was investigated by incubate the inoculated production media at different degree of temperature, pH, incubation period ,at the ordinary and shacking incubator, and by add (Glycine, Tyrosine ,Lysine ,Alanine) in two Con.
0.5mg/L and 1mg/L.
The result show that all these factor affecting on the production of S-layer protein and the highest protein concentration was obtained at temperature 37 ºc, pH 7, after incubation period 24 hrs., at shacking incubator, by add1mg/L of glycine
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Nuri, Lubna Adil& Taj al-Din, Wijdan R.& Abd, Firyal Jamil. 2016. Physiological study for extracted surface layer from aeromonas hydrophila isolated from diarrhea. Mesopotamia Environment Journal،Vol. 2, no. 3, pp.11-26.
https://search.emarefa.net/detail/BIM-693851
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Nuri, Lubna Adil…[et al.]. Physiological study for extracted surface layer from aeromonas hydrophila isolated from diarrhea. Mesopotamia Environment Journal Vol. 2, no. 3 (May. 2016), pp.11-26.
https://search.emarefa.net/detail/BIM-693851
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Nuri, Lubna Adil& Taj al-Din, Wijdan R.& Abd, Firyal Jamil. Physiological study for extracted surface layer from aeromonas hydrophila isolated from diarrhea. Mesopotamia Environment Journal. 2016. Vol. 2, no. 3, pp.11-26.
https://search.emarefa.net/detail/BIM-693851
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 22-26
رقم السجل
BIM-693851
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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