Physiological study for extracted surface layer from aeromonas hydrophila isolated from diarrhea
Joint Authors
Abd, Firyal Jamil
al-Nuri, Lubna Adil
Taj al-Din, Wijdan R.
Source
Mesopotamia Environment Journal
Issue
Vol. 2, Issue 3 (31 May. 2016), pp.11-26, 16 p.
Publisher
University of Babylon Environmental Research and Studies Center
Publication Date
2016-05-31
Country of Publication
Iraq
No. of Pages
16
Main Subjects
Topics
Abstract EN
A total of 349 stool samples were collected from the patients with diarrhea during the period from December 2014 to May 2015,the results show that there were 11(3.15%) positive isolates of Aeromonas hydrophila, these isolates were identified by cultural method ,biochemical tests and confirmed by API 20E and Vitek 2 system.Tryptose soy agar that contained Congo red stain was used to detect A.hydrophila isolates that produced surface layer protein (S-layer) ,eight (8) isolates was given positive result on this media.
The S-layer protein was extracted by two methods, Sodium Dodecyl Sulphate (SDS 0.05%) solution was given significantly higher protein concentration in compared with LiCl 5M solution.
Then it was precipitated by two methods also, ammonium per sulfate 80% was given significantly higher protein concentration in compared with acetone Con.95%.
The molecular weight of S-layer protein was determined by SDS-PAGE and the result reveal that it was 52 KDa.
The physiological factors that affecting on the production of S-layer protein from A.hydrophila was investigated by incubate the inoculated production media at different degree of temperature, pH, incubation period ,at the ordinary and shacking incubator, and by add (Glycine, Tyrosine ,Lysine ,Alanine) in two Con.
0.5mg/L and 1mg/L.
The result show that all these factor affecting on the production of S-layer protein and the highest protein concentration was obtained at temperature 37 ºc, pH 7, after incubation period 24 hrs., at shacking incubator, by add1mg/L of glycine
American Psychological Association (APA)
al-Nuri, Lubna Adil& Taj al-Din, Wijdan R.& Abd, Firyal Jamil. 2016. Physiological study for extracted surface layer from aeromonas hydrophila isolated from diarrhea. Mesopotamia Environment Journal،Vol. 2, no. 3, pp.11-26.
https://search.emarefa.net/detail/BIM-693851
Modern Language Association (MLA)
al-Nuri, Lubna Adil…[et al.]. Physiological study for extracted surface layer from aeromonas hydrophila isolated from diarrhea. Mesopotamia Environment Journal Vol. 2, no. 3 (May. 2016), pp.11-26.
https://search.emarefa.net/detail/BIM-693851
American Medical Association (AMA)
al-Nuri, Lubna Adil& Taj al-Din, Wijdan R.& Abd, Firyal Jamil. Physiological study for extracted surface layer from aeromonas hydrophila isolated from diarrhea. Mesopotamia Environment Journal. 2016. Vol. 2, no. 3, pp.11-26.
https://search.emarefa.net/detail/BIM-693851
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 22-26
Record ID
BIM-693851