Cytotoxicity and cytogenetic effects of local purslane (portulaca oleracea)‎ leaf crude extracts on normal and cancer cell lines in vitro

الملخص EN

The present study was carried out to evaluate the cytotoxic effects of aqueous and ethanol crude extracts of the leaf of Portulaca oleracea L.

against two malignant cell lines which were Murine mammary adenocarcinoma (AMN3), human Rhabdomyosarcoma (RD) and one normal cell line which was kidney epithelium of African green monkey (Vero).

Also the study included the cytogenetic effects of aqueous and ethanol crude extracts of P.

oleracea leaf on the mitotic index of Vero, AMN3 and RD cell lines In vitro.

After plant sample treatment were observed and have reported through several assays such as trypan blue exlusion assay for cell viability, cytotoxicity assay by crystal violet and mitotic index assay.

The aqueous crude extract was found to be more effective antiproliferate agent than the ethanol crude extract.

Both extracts exhibited time-dependent cytotoxic effects against AMN3 and RD cancer cell lines.

In which AMN3 was more sensitive to the extracts than RD.

All concentrations of aqueous extract were affected AMN3 cell line at 72hrs.

of exposure, but for ethanol extract the last dose (0.01μg/ ml) was not effective.

While the Vero normal cell line showed resistance toward all concentrations of both extracts except the first dose (10000μg/ ml), at all time of exposure (24, 48 and 72)hrs.