Cytotoxicity and cytogenetic effects of local purslane (portulaca oleracea) leaf crude extracts on normal and cancer cell lines in vitro
Joint Authors
Shaykhah, Zakariyya Ahmad
Hidayat, Hazha Jamal
Source
ZANCO Journal of Pure and Applied Sciences
Issue
Vol. 28, Issue 3 (30 Sep. 2016), pp.161-174, 14 p.
Publisher
Salahaddin University-Erbil Department of Scientific Publications
Publication Date
2016-09-30
Country of Publication
Iraq
No. of Pages
14
Main Subjects
Abstract EN
The present study was carried out to evaluate the cytotoxic effects of aqueous and ethanol crude extracts of the leaf of Portulaca oleracea L.
against two malignant cell lines which were Murine mammary adenocarcinoma (AMN3), human Rhabdomyosarcoma (RD) and one normal cell line which was kidney epithelium of African green monkey (Vero).
Also the study included the cytogenetic effects of aqueous and ethanol crude extracts of P.
oleracea leaf on the mitotic index of Vero, AMN3 and RD cell lines In vitro.
After plant sample treatment were observed and have reported through several assays such as trypan blue exlusion assay for cell viability, cytotoxicity assay by crystal violet and mitotic index assay.
The aqueous crude extract was found to be more effective antiproliferate agent than the ethanol crude extract.
Both extracts exhibited time-dependent cytotoxic effects against AMN3 and RD cancer cell lines.
In which AMN3 was more sensitive to the extracts than RD.
All concentrations of aqueous extract were affected AMN3 cell line at 72hrs.
of exposure, but for ethanol extract the last dose (0.01μg/ ml) was not effective.
While the Vero normal cell line showed resistance toward all concentrations of both extracts except the first dose (10000μg/ ml), at all time of exposure (24, 48 and 72)hrs.
American Psychological Association (APA)
Shaykhah, Zakariyya Ahmad& Hidayat, Hazha Jamal. 2016. Cytotoxicity and cytogenetic effects of local purslane (portulaca oleracea) leaf crude extracts on normal and cancer cell lines in vitro. ZANCO Journal of Pure and Applied Sciences،Vol. 28, no. 3, pp.161-174.
https://search.emarefa.net/detail/BIM-708847
Modern Language Association (MLA)
Shaykhah, Zakariyya Ahmad& Hidayat, Hazha Jamal. Cytotoxicity and cytogenetic effects of local purslane (portulaca oleracea) leaf crude extracts on normal and cancer cell lines in vitro. ZANCO Journal of Pure and Applied Sciences Vol. 28, no. 3 (2016), pp.161-174.
https://search.emarefa.net/detail/BIM-708847
American Medical Association (AMA)
Shaykhah, Zakariyya Ahmad& Hidayat, Hazha Jamal. Cytotoxicity and cytogenetic effects of local purslane (portulaca oleracea) leaf crude extracts on normal and cancer cell lines in vitro. ZANCO Journal of Pure and Applied Sciences. 2016. Vol. 28, no. 3, pp.161-174.
https://search.emarefa.net/detail/BIM-708847
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 173-174
Record ID
BIM-708847