Prevalence of methicillin resistant staphylococcus aureus (mrsa)‎ in molouha (salted fish)‎

الملخص EN

Fifty samples of Salted Hydrocynus Froskalli Fish (Molouha) were subjected for enumeration, isolation and identification of Staph.aureus.

34 strains (68%) of Staph.aureus were isolated, 16 (32%) were coagulase positive and 18 (36%) were coagulase negative, the mean Staph.aureus count was 35x103 cfu/g.

It was found that 46% of examined samples were unaccepted based on the Egyptian Organization for standardization and quality control.

The antimicrobial sensitivity revealed that isolated coagulase positive Staph.aureus were resistance to Neomycin, Penicillin, Kanamycin, Ampicillin, Chloramphenicol, Erythromycin, Oxytetracycline, Cloxacillin, Sulphamethoxazol, Ciprofloxacin, Cephalothin, Gentamicin, Methicillin and Vancomycin with a percentage of 100, 100, 93.7, 81.3, 75.0, 62.5, 56.3, 56.3, 50.0, 43.7, 37.5, 25.0, 25.0 and 6.3% respectively, whereas the high resistance rate was observed against Neomycin and Penicillin (100%) and the isolates showed 11 different patterns of antimicrobial sensitivity, whereas the high sensitive rate was observed with vancomycin (87.5%).

Detection of mecA gene was done for all coagulase positive Staph.aureus strains (16) by PCR using specific primer with amplification length of 533 pb.

The obtained results showed that all (MRSA) isolates were positive for presence of mecA gene and one of these isolets could not produce enterotoxin, whereas two could produce SEA and one could produce SEC.