Prevalence of methicillin resistant staphylococcus aureus (mrsa) in molouha (salted fish)
Other Title(s)
مدى تواجد ميكروب المكور العنقودي الذهبي المقاوم للميثيثلين في الملوحة
Joint Authors
Ibrahim, Lubna Muhammad
Thabit, Manal Hasan
Source
Assiut Veterinary Medical Journal
Issue
Vol. 62, Issue 150 (31 Jul. 2016), pp.107-114, 8 p.
Publisher
Assiut University Faculty of Veterinary Medicine
Publication Date
2016-07-31
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Topics
- Fishes
- Salinity
- Egypt
- Antibacterial agents
- Drug resistance
- Staphylococcus
- Diagnosis
- Molecular aspects
- Methicillin
Abstract EN
Fifty samples of Salted Hydrocynus Froskalli Fish (Molouha) were subjected for enumeration, isolation and identification of Staph.aureus.
34 strains (68%) of Staph.aureus were isolated, 16 (32%) were coagulase positive and 18 (36%) were coagulase negative, the mean Staph.aureus count was 35x103 cfu/g.
It was found that 46% of examined samples were unaccepted based on the Egyptian Organization for standardization and quality control.
The antimicrobial sensitivity revealed that isolated coagulase positive Staph.aureus were resistance to Neomycin, Penicillin, Kanamycin, Ampicillin, Chloramphenicol, Erythromycin, Oxytetracycline, Cloxacillin, Sulphamethoxazol, Ciprofloxacin, Cephalothin, Gentamicin, Methicillin and Vancomycin with a percentage of 100, 100, 93.7, 81.3, 75.0, 62.5, 56.3, 56.3, 50.0, 43.7, 37.5, 25.0, 25.0 and 6.3% respectively, whereas the high resistance rate was observed against Neomycin and Penicillin (100%) and the isolates showed 11 different patterns of antimicrobial sensitivity, whereas the high sensitive rate was observed with vancomycin (87.5%).
Detection of mecA gene was done for all coagulase positive Staph.aureus strains (16) by PCR using specific primer with amplification length of 533 pb.
The obtained results showed that all (MRSA) isolates were positive for presence of mecA gene and one of these isolets could not produce enterotoxin, whereas two could produce SEA and one could produce SEC.
American Psychological Association (APA)
Ibrahim, Lubna Muhammad& Thabit, Manal Hasan. 2016. Prevalence of methicillin resistant staphylococcus aureus (mrsa) in molouha (salted fish). Assiut Veterinary Medical Journal،Vol. 62, no. 150, pp.107-114.
https://search.emarefa.net/detail/BIM-709010
Modern Language Association (MLA)
Ibrahim, Lubna Muhammad& Thabit, Manal Hasan. Prevalence of methicillin resistant staphylococcus aureus (mrsa) in molouha (salted fish). Assiut Veterinary Medical Journal Vol. 62, no. 150 (Jul. 2016), pp.107-114.
https://search.emarefa.net/detail/BIM-709010
American Medical Association (AMA)
Ibrahim, Lubna Muhammad& Thabit, Manal Hasan. Prevalence of methicillin resistant staphylococcus aureus (mrsa) in molouha (salted fish). Assiut Veterinary Medical Journal. 2016. Vol. 62, no. 150, pp.107-114.
https://search.emarefa.net/detail/BIM-709010
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 111-113
Record ID
BIM-709010