Optimization of cellulase enzyme production from Trichoderma isolates under submerged fermentation
العناوين الأخرى
الظروف المثلى لإنتاج أنزيم السيلوليز من عزلات الترايكوديرما بواسطة التخمر المغمور
مقدم أطروحة جامعية
مشرف أطروحة جامعية
al-Bashiti, Tariq Abd al-Qadir
أعضاء اللجنة
al-Qishawi, Abbud Yasir
al-Lahham, Nahid Ali
الجامعة
الجامعة الإسلامية
الكلية
كلية العلوم
القسم الأكاديمي
قسم العلوم الحياتية
دولة الجامعة
فلسطين (قطاع غزة)
الدرجة العلمية
ماجستير
تاريخ الدرجة العلمية
2015
الملخص الإنجليزي
Cellulases are enzymes that analyze the biomass of the cellulose and it is used in many fields such as the paper industry, fabric yarn, manufacture of detergents and laundry.
Cellulase enzyme is produced by micro-organisms that grow on cellulosic materials.
The main purpose of this study is to find out the optimal conditions for the production of cellulase enzyme by the local Trichoderma isolates.
In this study, Trichoderma was isolated from air and soil of the Islamic University gaza campus male and female.
Three Trichoderma species were isolated and identified as following Trichoderma harizanium, Trichoderma reesi and Trichoderma viride.
Congo red and CMC have been used to know the best species of Trichoderm have ability to produce cellulase enzyme and Trichoderma viride was the best for cellulase production.
The produced amount of glucose was examined by cellulase enzyme that produced by Trichoderma viride using glucose kit assay.
The optimum conditions for cellulase enzyme production were found.
The required carbon and nitrogen sources and the environmental conditions such as pH and temperature were optimized.
The results showed that the best activity for cellulase enzyme is at 7 days in basal salt media where the produced amount of glucose reached (30.8 mmol / L), pH of 5.5 and a temperature of 30 °C.
The best source of carbon and nitrogen to produce cellulase enzyme are 1% sucrose and 1% yeast extract respectively.
Trichoderma viride is considered a unique source for the production of cellulase enzyme in large quantities compared to other Trichoderma species.
التخصصات الرئيسية
عدد الصفحات
57
قائمة المحتويات
Table of contents.
Abstract.
Abstract in Arabic.
Chapter One : Introduction.
Chapter Two : Literature review.
Chapter Three : Materials and methods.
Chapter Four : Results.
Chapter Five : Discussion.
Chapter Six : Conclusion and recommendation.
References.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Budayr, Riham Jawad Fayiz. (2015). Optimization of cellulase enzyme production from Trichoderma isolates under submerged fermentation. (Master's theses Theses and Dissertations Master). Islamic University, Palestine (Gaza Strip)
https://search.emarefa.net/detail/BIM-724355
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Budayr, Riham Jawad Fayiz. Optimization of cellulase enzyme production from Trichoderma isolates under submerged fermentation. (Master's theses Theses and Dissertations Master). Islamic University. (2015).
https://search.emarefa.net/detail/BIM-724355
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Budayr, Riham Jawad Fayiz. (2015). Optimization of cellulase enzyme production from Trichoderma isolates under submerged fermentation. (Master's theses Theses and Dissertations Master). Islamic University, Palestine (Gaza Strip)
https://search.emarefa.net/detail/BIM-724355
لغة النص
الإنجليزية
نوع البيانات
رسائل جامعية
رقم السجل
BIM-724355
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر