Optimization of cellulase enzyme production from Trichoderma isolates under submerged fermentation
Other Title(s)
الظروف المثلى لإنتاج أنزيم السيلوليز من عزلات الترايكوديرما بواسطة التخمر المغمور
Dissertant
Thesis advisor
al-Bashiti, Tariq Abd al-Qadir
Comitee Members
al-Qishawi, Abbud Yasir
al-Lahham, Nahid Ali
University
Islamic University
Faculty
Faculty of Science
Department
Department of Biological Science
University Country
Palestine (Gaza Strip)
Degree
Master
Degree Date
2015
English Abstract
Cellulases are enzymes that analyze the biomass of the cellulose and it is used in many fields such as the paper industry, fabric yarn, manufacture of detergents and laundry.
Cellulase enzyme is produced by micro-organisms that grow on cellulosic materials.
The main purpose of this study is to find out the optimal conditions for the production of cellulase enzyme by the local Trichoderma isolates.
In this study, Trichoderma was isolated from air and soil of the Islamic University gaza campus male and female.
Three Trichoderma species were isolated and identified as following Trichoderma harizanium, Trichoderma reesi and Trichoderma viride.
Congo red and CMC have been used to know the best species of Trichoderm have ability to produce cellulase enzyme and Trichoderma viride was the best for cellulase production.
The produced amount of glucose was examined by cellulase enzyme that produced by Trichoderma viride using glucose kit assay.
The optimum conditions for cellulase enzyme production were found.
The required carbon and nitrogen sources and the environmental conditions such as pH and temperature were optimized.
The results showed that the best activity for cellulase enzyme is at 7 days in basal salt media where the produced amount of glucose reached (30.8 mmol / L), pH of 5.5 and a temperature of 30 °C.
The best source of carbon and nitrogen to produce cellulase enzyme are 1% sucrose and 1% yeast extract respectively.
Trichoderma viride is considered a unique source for the production of cellulase enzyme in large quantities compared to other Trichoderma species.
Main Subjects
No. of Pages
57
Table of Contents
Table of contents.
Abstract.
Abstract in Arabic.
Chapter One : Introduction.
Chapter Two : Literature review.
Chapter Three : Materials and methods.
Chapter Four : Results.
Chapter Five : Discussion.
Chapter Six : Conclusion and recommendation.
References.
American Psychological Association (APA)
Budayr, Riham Jawad Fayiz. (2015). Optimization of cellulase enzyme production from Trichoderma isolates under submerged fermentation. (Master's theses Theses and Dissertations Master). Islamic University, Palestine (Gaza Strip)
https://search.emarefa.net/detail/BIM-724355
Modern Language Association (MLA)
Budayr, Riham Jawad Fayiz. Optimization of cellulase enzyme production from Trichoderma isolates under submerged fermentation. (Master's theses Theses and Dissertations Master). Islamic University. (2015).
https://search.emarefa.net/detail/BIM-724355
American Medical Association (AMA)
Budayr, Riham Jawad Fayiz. (2015). Optimization of cellulase enzyme production from Trichoderma isolates under submerged fermentation. (Master's theses Theses and Dissertations Master). Islamic University, Palestine (Gaza Strip)
https://search.emarefa.net/detail/BIM-724355
Language
English
Data Type
Arab Theses
Record ID
BIM-724355
