Dose-response curves of the FDXR and RAD51 genes with 6 and 18 MV beam energies in human peripheral blood lymphocytes
المؤلفون المشاركون
Sabri, Alihusayn
Khodamoradi, Ihsan
Birgani, Muhammad Javad Tahmasebi
Makvandi, Manoochehr
Nuri, Bijan
المصدر
Iranian Red Crescent Medical Journal
العدد
المجلد 18، العدد 11 (30 نوفمبر/تشرين الثاني 2016)، ص ص. 1-8، 8ص.
الناشر
تاريخ النشر
2016-11-30
دولة النشر
الإمارات العربية المتحدة
عدد الصفحات
8
التخصصات الرئيسية
العلوم الطبية والصيدلة والعلوم الصحية
الملخص EN
Background : Rapid dose assessment using biological dosimetry methods is essential to increase the chance of survival of exposed individuals in radiation accidents.
Objectives : We compared the expression levels of the FDXR and RAD51 genes at 6 and 18 MV beam energies in human peripheral blood lymphocytes.
The results of our study can be used to analyze radiation energy in biological dosimetry.
Methods : For this in vitro experimental study, from 36 students in the medical physics and virology departments, seven voluntary, healthy, non-smoking male blood donors of Khuzestan ethnicity with no history of exposure to ionization radiation were selected using simple randomized sampling.
Sixty-three peripheral blood samples were collected from the seven healthy donors.
Human peripheral bloodwasthen exposed to doses of 0, 0.2, 0.5, 2, and4Gywith6and18MVbeamenergies in a LinacVarian 2100C/D (Varian, USA) at Golestan hospital in Ahvaz, Iran.
After RNA extraction and cDNA synthesis, the expression levels of FDXR and RAD51 were determined 24 hours post-irradiation using the gel-purified reverse transcription polymerase chain reaction (RT-PCR) technique and TaqMan strategy (by real-time PCR).
Results: The expression level of FDXR genewassignificantly increased at doses of 2Gyand4Gyin the 6 - 18MVenergy range (P< 0.001 and P < 0.008, respectively).
The medians with interquartile ranges (IQRs) of the copy numbers of the FDXR gene at 2 Gy and 4 Gy doses under 6 and 18 MV beam energies were 2393.59 (1798.21, 2575.37) and 2983.00 (2199.48, 3643.82) and 3779.12 (3051.40, 5120.74) and 5051.26 (4704.83, 5859.17), respectively.
However, RAD51 gene expression levels only showed a significant difference between samples at a dose of 2 Gy with 6 and 18 MV beam energies, respectively (P < 0.040).
The medians with IQRs of the copy numbers of the RAD51 gene were 2092.77 (1535.78, 2705.61) and 3412.57 (2979.72, 4530.61) at beam energies of 6 and 18 MV, respectively.
Conclusions : The data suggest that the expression analysis of the FDXR gene, contrary to that of the RAD51 gene, may be suitable for assessment of high-energy X-ray.
In addition, RAD51 is not a suitable gene for dose assessment in biological dosimetry.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sabri, Alihusayn& Khodamoradi, Ihsan& Birgani, Muhammad Javad Tahmasebi& Makvandi, Manoochehr& Nuri, Bijan. 2016. Dose-response curves of the FDXR and RAD51 genes with 6 and 18 MV beam energies in human peripheral blood lymphocytes. Iranian Red Crescent Medical Journal،Vol. 18, no. 11, pp.1-8.
https://search.emarefa.net/detail/BIM-728752
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sabri, Alihusayn…[et al.]. Dose-response curves of the FDXR and RAD51 genes with 6 and 18 MV beam energies in human peripheral blood lymphocytes. Iranian Red Crescent Medical Journal Vol. 18, no. 11 (Nov. 2016), pp.1-8.
https://search.emarefa.net/detail/BIM-728752
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sabri, Alihusayn& Khodamoradi, Ihsan& Birgani, Muhammad Javad Tahmasebi& Makvandi, Manoochehr& Nuri, Bijan. Dose-response curves of the FDXR and RAD51 genes with 6 and 18 MV beam energies in human peripheral blood lymphocytes. Iranian Red Crescent Medical Journal. 2016. Vol. 18, no. 11, pp.1-8.
https://search.emarefa.net/detail/BIM-728752
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 8
رقم السجل
BIM-728752
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر