Dose-response curves of the FDXR and RAD51 genes with 6 and 18 MV beam energies in human peripheral blood lymphocytes

الملخص EN

Background : Rapid dose assessment using biological dosimetry methods is essential to increase the chance of survival of exposed individuals in radiation accidents.

Objectives : We compared the expression levels of the FDXR and RAD51 genes at 6 and 18 MV beam energies in human peripheral blood lymphocytes.

The results of our study can be used to analyze radiation energy in biological dosimetry.

Methods : For this in vitro experimental study, from 36 students in the medical physics and virology departments, seven voluntary, healthy, non-smoking male blood donors of Khuzestan ethnicity with no history of exposure to ionization radiation were selected using simple randomized sampling.

Sixty-three peripheral blood samples were collected from the seven healthy donors.

Human peripheral bloodwasthen exposed to doses of 0, 0.2, 0.5, 2, and4Gywith6and18MVbeamenergies in a LinacVarian 2100C/D (Varian, USA) at Golestan hospital in Ahvaz, Iran.

After RNA extraction and cDNA synthesis, the expression levels of FDXR and RAD51 were determined 24 hours post-irradiation using the gel-purified reverse transcription polymerase chain reaction (RT-PCR) technique and TaqMan strategy (by real-time PCR).

Results: The expression level of FDXR genewassignificantly increased at doses of 2Gyand4Gyin the 6 - 18MVenergy range (P< 0.001 and P < 0.008, respectively).

The medians with interquartile ranges (IQRs) of the copy numbers of the FDXR gene at 2 Gy and 4 Gy doses under 6 and 18 MV beam energies were 2393.59 (1798.21, 2575.37) and 2983.00 (2199.48, 3643.82) and 3779.12 (3051.40, 5120.74) and 5051.26 (4704.83, 5859.17), respectively.

However, RAD51 gene expression levels only showed a significant difference between samples at a dose of 2 Gy with 6 and 18 MV beam energies, respectively (P < 0.040).

The medians with IQRs of the copy numbers of the RAD51 gene were 2092.77 (1535.78, 2705.61) and 3412.57 (2979.72, 4530.61) at beam energies of 6 and 18 MV, respectively.

Conclusions : The data suggest that the expression analysis of the FDXR gene, contrary to that of the RAD51 gene, may be suitable for assessment of high-energy X-ray.

In addition, RAD51 is not a suitable gene for dose assessment in biological dosimetry.