Dose-response curves of the FDXR and RAD51 genes with 6 and 18 MV beam energies in human peripheral blood lymphocytes
Joint Authors
Sabri, Alihusayn
Khodamoradi, Ihsan
Birgani, Muhammad Javad Tahmasebi
Makvandi, Manoochehr
Nuri, Bijan
Source
Iranian Red Crescent Medical Journal
Issue
Vol. 18, Issue 11 (30 Nov. 2016), pp.1-8, 8 p.
Publisher
Publication Date
2016-11-30
Country of Publication
United Arab Emirates
No. of Pages
8
Main Subjects
Pharmacy, Health & Medical Sciences
Abstract EN
Background : Rapid dose assessment using biological dosimetry methods is essential to increase the chance of survival of exposed individuals in radiation accidents.
Objectives : We compared the expression levels of the FDXR and RAD51 genes at 6 and 18 MV beam energies in human peripheral blood lymphocytes.
The results of our study can be used to analyze radiation energy in biological dosimetry.
Methods : For this in vitro experimental study, from 36 students in the medical physics and virology departments, seven voluntary, healthy, non-smoking male blood donors of Khuzestan ethnicity with no history of exposure to ionization radiation were selected using simple randomized sampling.
Sixty-three peripheral blood samples were collected from the seven healthy donors.
Human peripheral bloodwasthen exposed to doses of 0, 0.2, 0.5, 2, and4Gywith6and18MVbeamenergies in a LinacVarian 2100C/D (Varian, USA) at Golestan hospital in Ahvaz, Iran.
After RNA extraction and cDNA synthesis, the expression levels of FDXR and RAD51 were determined 24 hours post-irradiation using the gel-purified reverse transcription polymerase chain reaction (RT-PCR) technique and TaqMan strategy (by real-time PCR).
Results: The expression level of FDXR genewassignificantly increased at doses of 2Gyand4Gyin the 6 - 18MVenergy range (P< 0.001 and P < 0.008, respectively).
The medians with interquartile ranges (IQRs) of the copy numbers of the FDXR gene at 2 Gy and 4 Gy doses under 6 and 18 MV beam energies were 2393.59 (1798.21, 2575.37) and 2983.00 (2199.48, 3643.82) and 3779.12 (3051.40, 5120.74) and 5051.26 (4704.83, 5859.17), respectively.
However, RAD51 gene expression levels only showed a significant difference between samples at a dose of 2 Gy with 6 and 18 MV beam energies, respectively (P < 0.040).
The medians with IQRs of the copy numbers of the RAD51 gene were 2092.77 (1535.78, 2705.61) and 3412.57 (2979.72, 4530.61) at beam energies of 6 and 18 MV, respectively.
Conclusions : The data suggest that the expression analysis of the FDXR gene, contrary to that of the RAD51 gene, may be suitable for assessment of high-energy X-ray.
In addition, RAD51 is not a suitable gene for dose assessment in biological dosimetry.
American Psychological Association (APA)
Sabri, Alihusayn& Khodamoradi, Ihsan& Birgani, Muhammad Javad Tahmasebi& Makvandi, Manoochehr& Nuri, Bijan. 2016. Dose-response curves of the FDXR and RAD51 genes with 6 and 18 MV beam energies in human peripheral blood lymphocytes. Iranian Red Crescent Medical Journal،Vol. 18, no. 11, pp.1-8.
https://search.emarefa.net/detail/BIM-728752
Modern Language Association (MLA)
Sabri, Alihusayn…[et al.]. Dose-response curves of the FDXR and RAD51 genes with 6 and 18 MV beam energies in human peripheral blood lymphocytes. Iranian Red Crescent Medical Journal Vol. 18, no. 11 (Nov. 2016), pp.1-8.
https://search.emarefa.net/detail/BIM-728752
American Medical Association (AMA)
Sabri, Alihusayn& Khodamoradi, Ihsan& Birgani, Muhammad Javad Tahmasebi& Makvandi, Manoochehr& Nuri, Bijan. Dose-response curves of the FDXR and RAD51 genes with 6 and 18 MV beam energies in human peripheral blood lymphocytes. Iranian Red Crescent Medical Journal. 2016. Vol. 18, no. 11, pp.1-8.
https://search.emarefa.net/detail/BIM-728752
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 8
Record ID
BIM-728752