Optimized and validated spectrophotometric methods for the determination of amiodarone hydrochloride in commercial dosage forms using N-bromosuccinimide and bromothymol blue

الملخص EN

Two optimized and validated spectrophotometric methods have been developed for the determination of amiodarone hydrochloride in commercial dosage forms.

Method A is based on the reaction of tertiary amino group of the drug with N-bromosuccinimide in methanol-acetone medium resulting in the formation of a yellow coloredproduct, which absorbed maximally at 353 nm.

Method B involves the formation of a colored chloroform extractable ion-pair complex of the drug with bromothymol blue at pH 2.32 absorbing maximally at 400 nm.

Beer’s law is obeyed in the concentration ranges 50-600 and 2-55 ig mL^1 with molar absorptivity of 1.58 x 102 and 1.50 x 104 Lmol^1 cm^1 for methods A and B, respectively.

The regression analysis yields the calibration equations: A = 4.012 X 10^4 + 1.16 X 10^3 C and A = 9.612; 10 C for methods A and B, respectively.

The application of the proposed methods to commercial dosage forms is presented.

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