شروط استخلاص الكولاجين من جلود كل من الأبقار و الأغنام و الدجاج و المقارنة بينها

الملخص EN

The aim of this research is the determination of the appropriate conditions for extracting collagen by acidic method from remnants of skins of sheep, cows, and chickens.

The results showed that acetic acid is the best among the various organic acids, for extracting collagen of these skins during 48 hours, at concentration of 0.5 M and the ratio, acidic: skin (6:1).

NaOH solution has been used for 24 hours, at concentration of 0.1 M and the ratio, alkaline: skin (3:1), for extracting Collagen proteins from raw material, by repeating the process 5 times.

To get rid of the raw material fat, anhydrous n-butanol (3:1) has been used on three batches, during 24 hours.

The dialysis method has been used for collagen purification, where the percentage of pure collagen reached 10.5%, 6%, and 2% in remnants of skins of chickens, sheep, and cows, respectively