leptin gene snps and their effect on milk traits in Egyptian buffalo (Bubalus Bubalis)‎

الملخص EN

Leptin plays an important role in the regulation of development of the mammary gland and its polymorphisms were shown to be associated with milk quantity and quality in cattle.

However, no SNP was detected and no association studies was conducted on leptin gene in Egyptian buffalo.

Therefore, the objective of this study was to detect leptin gene polymorphisms and to determine associations between these polymorphisms and milk traits in Egyptian water buffalo.

A PCR product of 400bp, including intron 2 of leptin gene was amplified using PCR and subsequently, subjected to sequence analysis to identify its different allelic patterns.

Two novel SNPs,G247T at nucleotide number 963 and T282G at nucleotide number 998, were detected in LEP intron 2 in Egyptian buffaloes.

The associations analysis revealed no significance association between these SNPs and milk traits(P>0.05) in all examined animals.

Consequently, our results indicated that the LEPG247T and T282GSNPs does not influence milk yield or milk composition in Egyptian buffalo and these SNPs could not be used in a marker assisted selection program to improve milk traits in Egyptian buffalo