leptin gene snps and their effect on milk traits in Egyptian buffalo (Bubalus Bubalis)
Joint Authors
Abduh, Safa al-Sayyid
Abu al-Majd, Muhammad
al-Nahhas, Abir Fikri
Mansur, Ali Abd al-Qadir
Source
Kafr El-Sheikh Veterinary Medical Journal
Issue
Vol. 12, Issue 1 (30 Jun. 2014), pp.97-110, 14 p.
Publisher
Kafr El-Sheikh University Faculty of Veterinary Medicine
Publication Date
2014-06-30
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Leptin plays an important role in the regulation of development of the mammary gland and its polymorphisms were shown to be associated with milk quantity and quality in cattle.
However, no SNP was detected and no association studies was conducted on leptin gene in Egyptian buffalo.
Therefore, the objective of this study was to detect leptin gene polymorphisms and to determine associations between these polymorphisms and milk traits in Egyptian water buffalo.
A PCR product of 400bp, including intron 2 of leptin gene was amplified using PCR and subsequently, subjected to sequence analysis to identify its different allelic patterns.
Two novel SNPs,G247T at nucleotide number 963 and T282G at nucleotide number 998, were detected in LEP intron 2 in Egyptian buffaloes.
The associations analysis revealed no significance association between these SNPs and milk traits(P>0.05) in all examined animals.
Consequently, our results indicated that the LEPG247T and T282GSNPs does not influence milk yield or milk composition in Egyptian buffalo and these SNPs could not be used in a marker assisted selection program to improve milk traits in Egyptian buffalo
American Psychological Association (APA)
Abduh, Safa al-Sayyid& Abu al-Majd, Muhammad& al-Nahhas, Abir Fikri& Mansur, Ali Abd al-Qadir. 2014. leptin gene snps and their effect on milk traits in Egyptian buffalo (Bubalus Bubalis). Kafr El-Sheikh Veterinary Medical Journal،Vol. 12, no. 1, pp.97-110.
https://search.emarefa.net/detail/BIM-761339
Modern Language Association (MLA)
Abduh, Safa al-Sayyid…[et al.]. leptin gene snps and their effect on milk traits in Egyptian buffalo (Bubalus Bubalis). Kafr El-Sheikh Veterinary Medical Journal Vol. 12, no. 1 (2014), pp.97-110.
https://search.emarefa.net/detail/BIM-761339
American Medical Association (AMA)
Abduh, Safa al-Sayyid& Abu al-Majd, Muhammad& al-Nahhas, Abir Fikri& Mansur, Ali Abd al-Qadir. leptin gene snps and their effect on milk traits in Egyptian buffalo (Bubalus Bubalis). Kafr El-Sheikh Veterinary Medical Journal. 2014. Vol. 12, no. 1, pp.97-110.
https://search.emarefa.net/detail/BIM-761339
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 107-110
Record ID
BIM-761339