Molecular study of SHV-11 and SHV-12 genes among klebsiella pneumonia isolated from UTI patients in Erbil City
المؤلفون المشاركون
Tawgozi, Fayruz H. Abd Allah
Amin, Bushra K.
المصدر
ZANCO Journal of Pure and Applied Sciences
العدد
المجلد 28، العدد 4 (31 أغسطس/آب 2016)، ص ص. 25-35، 11ص.
الناشر
جامعة صلاح الدين قسم النشر العلمي
تاريخ النشر
2016-08-31
دولة النشر
العراق
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Among 350 urine samples collected from urinary tract infection patients in Erbil city, (50) isolates were identified as Klebsiella pneumoniae using cultural, morphological and biochemical characteristics.
Their identity confirmed by Vitek2 system.
Extended-spectrum β-lactamase (ESBL) production determined by phenotypic and molecular methods.
Phenotypic detection methods include an initial screening by using (ATM30, CTX5 and CI30), by which (52%) were resistant.
Phenotypic ESBL production was confirmed by DDST and CDM.
Eight per cent of the isolates were ESBL producer by DDST and (42%) were ESBL producer by CDM.
Fifty per cent of the isolates were ESBL producer by both methods.
Polymerase Chain Reaction (PCR) amplification used for detection of SHV-11 (a non- ESBL) gene in the genomic DNA and SHV-12 (an ESBL) gene in the plasmid DNA.
SHV-11 found in the genomic DNA of 96% of ESBL producer isolates, while 88% of phenotypic ESBL negative isolates contained SHV-11 in their genomic DNA.
Seventy two per cent of the phenotypic ESBL producer isolates had SHV-12 in their plasmid DNA, whereas 44% of the phenotypic ESBL negative isolates contained SHV-12 in their plasmid DNA.
Out of 25 phenotypic ESBL producer isolates, 72% carried SHV-11 and SHV-12, whilst only 44% of phenotypic ESBL negative isolates contained both genes.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Tawgozi, Fayruz H. Abd Allah& Amin, Bushra K.. 2016. Molecular study of SHV-11 and SHV-12 genes among klebsiella pneumonia isolated from UTI patients in Erbil City. ZANCO Journal of Pure and Applied Sciences،Vol. 28, no. 4, pp.25-35.
https://search.emarefa.net/detail/BIM-762107
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Tawgozi, Fayruz H. Abd Allah& Amin, Bushra K.. Molecular study of SHV-11 and SHV-12 genes among klebsiella pneumonia isolated from UTI patients in Erbil City. ZANCO Journal of Pure and Applied Sciences Vol. 28, no. 4 (2016), pp.25-35.
https://search.emarefa.net/detail/BIM-762107
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Tawgozi, Fayruz H. Abd Allah& Amin, Bushra K.. Molecular study of SHV-11 and SHV-12 genes among klebsiella pneumonia isolated from UTI patients in Erbil City. ZANCO Journal of Pure and Applied Sciences. 2016. Vol. 28, no. 4, pp.25-35.
https://search.emarefa.net/detail/BIM-762107
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 33-35
رقم السجل
BIM-762107
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر